Figure S5.
Panel A: Western blot images showing protein levels in stage 14 egg chambers across replicates, comparing wild-type and NASP conditions with input and aggregated fractions. The upper blots display anti-H3 and anti-H2B signals, while the lower panels show total protein as loading controls. Bands are organized by replicate and condition to illustrate differences in protein distribution between input and aggregate samples. Panel B: Western blot images showing protein levels in activated eggs across replicates, comparing wild-type and NASP conditions with input and aggregated fractions. The upper blots display anti-H3 and anti-H2B signals, while the lower panels show total protein as loading controls, highlighting differences in protein accumulation between conditions.
Western blots of histone aggregation across multiple replicates. (A) Western blot analysis for input and aggregate fractions from stage 14 egg chambers from WT and NASP mutant female flies used in quantification in Fig. 4 E (replicates 3 and 4). (B) Western blot analysis for input and aggregate fractions derived from activated eggs collected from WT or NASP-mutant females crossed to twine-mutant males used in quantification in Fig. 4 G. (Replicates 3 and 4). Source data are available for this figure: SourceData FS5.