Figure S3.
Panel A: Fluorescence microscopy images of DAPI-stained embryos from control and mutant crosses, showing differences in nuclear morphology and developmental progression, with higher-magnification insets highlighting representative regions. Scale bars indicate low- and high-magnification views. Panel B: A bar graph showing the percentage of embryos progressing beyond a specific developmental stage. The horizontal axis represents different genetic crosses, and the vertical axis represents the percentage of embryos progressing past the indicated nuclear cycle. Panel C: Western blot images showing protein levels in embryos from different genetic backgrounds, with multiple biological replicates. The horizontal arrangement represents different sample groups, and bands correspond to specific proteins detected, with loading controls included for comparison.
Embryos laid by WT females crossed to twine-mutant males fail to develop. (A) Representative images of DAPI-stained eggs from WT females crossed to WT males (WT X WT) and WT females crossed to twine-mutant males (WT X twine). Imaging was performed at 10X or 60X (yellow boxes). Scale bars represent 500 and 83 µm (inset). (B) Percentage of eggs laid that progress past nuclear cycle 10 from WT X WT and WT X twine-mutant crosses. (C) Western blot analysis of activated eggs collected from WT or NASP-mutant females crossed to twine-mutant males used in quantification in Fig. 4 B (replicates 3 and 4). Embryos indicate the number of embryos used for the western blots. Source data are available for this figure: SourceData FS3.