Figure 4.
H3m degradation happens only upon egg activation. (A) Western blot analysis of stage 14 egg chambers and activated eggs collected from WT or NASP-mutant females. (B) Quantification of H3 band intensity in WT and NASP stage 14 egg chambers and activated eggs. H3 protein levels are normalized to the total protein level. N = 3 biological replicates. Data represent the mean ± standard deviation (SD). Statistical difference between the groups was analyzed by employing a paired t test. (C) Western blot analysis of activated eggs collected from WT or NASP-mutant females crossed to twine-mutant males. Embryos represent the number of individual embryos used for preparing the sample. (D) Quantification of H3 and H2B protein levels in activated eggs laid by WT and NASP-mutant females crossed to twine-mutant males. Proteins levels are normalized to the WT values. N = 3 biological replicates. Data represent the mean ± standard deviation (SD). Statistical difference between the groups was analyzed by employing a paired t test. In the figure panels, ns represents nonsignificant difference, while *P < 0.05 and **P < 0.01. Source data are available for this figure: SourceData F4. Refer to the image caption for details. Panel A shows Western blot images from wild-type (WT) and NASP-mutant females across three replicates. The blots are probed with anti-H3 and total protein markers. Panel B presents two bar graphs quantifying H3 band intensity in stage 14 egg chambers and activated eggs. The y-axis represents H3 band intensity in arbitrary units (AU), and the x-axis compares WT and NASP-mutant samples. The left graph shows non-significant differences in egg chambers, while the right graph indicates a significant reduction in H3 levels in activated eggs from NASP-mutant females. Panel C displays Western blot images for activated eggs from WT and NASP-mutant females crossed to twine mutant males, with varying numbers of embryos. The blots are probed with anti-NASP and anti-histones markers for H3 and H2B. Panel D features two bar graphs quantifying H3 and H2B protein levels in activated eggs. The y-axis represents the percentage of protein levels normalized to WT values, and the x-axis compares WT and NASP-mutant samples. The left graph shows a significant reduction in H3 levels in NASP-mutant samples, while the right graph shows non-significant differences in H2B levels.

H3m degradation happens only upon egg activation. (A) Western blot analysis of stage 14 egg chambers and activated eggs collected from WT or NASP-mutant females. (B) Quantification of H3 band intensity in WT and NASP stage 14 egg chambers and activated eggs. H3 protein levels are normalized to the total protein level. N = 3 biological replicates. Data represent the mean ± standard deviation (SD). Statistical difference between the groups was analyzed by employing a paired t test. (C) Western blot analysis of activated eggs collected from WT or NASP-mutant females crossed to twine-mutant males. Embryos represent the number of individual embryos used for preparing the sample. (D) Quantification of H3 and H2B protein levels in activated eggs laid by WT and NASP-mutant females crossed to twine-mutant males. Proteins levels are normalized to the WT values. N = 3 biological replicates. Data represent the mean ± standard deviation (SD). Statistical difference between the groups was analyzed by employing a paired t test. In the figure panels, ns represents nonsignificant difference, while *P < 0.05 and **P < 0.01. Source data are available for this figure: SourceData F4.

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