Figure S2.
Panel A shows a PCR analysis of a gel electrophoresis image with lanes labeled WT, M1, M2, and M3. The WT lane displays bands at approximately 2000 base pairs (bp) and 5000 bp, while the M1, M2, and M3 lanes show shifted bands indicated by black arrowheads. Panel B presents a Western blot with lanes labeled Rep1, Rep2, and Rep3, each containing sub-lanes for heterozygous (het) and homozygous (homo) samples. The blot shows bands around 100 kilodaltons (kD) and 75 kD, with red and black arrowheads marking the Dendra-tagged NASP and wild-type NASP, respectively. Panel C is a scatter plot depicting H3.2-Dendra2 nuclear import rates across three nuclear cycles (11, 12, and 13). The y-axis represents the import rate in arbitrary units (AU), and the data points are color-coded: purple for total nuclear intensities and teal for non-chromatin associated intensities. The plot shows variability in import rates across the cycles.
Validation of NASP-Dendra fly line. (A) PCR analysis of the NASP endogenous locus shows ∼3 kb shift in the NASP-Dendra–tagged flies as compared with the WT flies. M1—male #1, M2—male #2, and M3—male #3. (B) Western blot analysis of ovary extracts from heterozygous (het) and homozygous (homo) NASP-Dendra female flies confirm the expression of NASP-Dendra (red arrowhead represents Dendra-tagged NASP, and the black arrowhead represents WT NASP). Three biological replicates are labelled as Rep1, Rep2, and Rep3. (C) H3.2 Dendra nuclear import rates are calculated from unnormalized total nuclear intensities (purple) and non-chromatin–associated intensities (teal). Source data are available for this figure: SourceData FS2.