Figure 1.
H3 nuclear import is independent of NASP. (A) Total intensity curves of H3.2-Dendra2 for nuclear cycles (NC) 11–13 in WT embryos. (B) Total intensity curves of H3.2-Dendra2 for nuclear cycles (NC) 11–13 in NASP-deficient embryos. (C) Nuclear import rates of H3.2-Dendra2 in WT (blue) and NASP-deficient (red) embryos. Simulated import rate for H3.2 at 50% WT levels (black). N = 6 biological replicates. Data represent the mean ± standard deviation (SD). Statistical difference between groups were analyzed by employing a two-way ANOVA Tukey’s multiple comparison test between the WT and NASP data points. (D) Total intensity curves of H3.3-Dendra2 for nuclear cycles (NC) 11–13 in WT embryos. (E) Total intensity curves of H3.3-Dendra2 for nuclear cycles (NC) 11–13 in NASP-deficient embryos. (F) Nuclear import rates of H3.2-Dendra2 in WT (blue) and NASP-deficient (red) embryos. N = 6 experimental replicates. Data represent the mean ± standard deviation (SD). Statistical difference between groups were analyzed by employing a two-way ANOVA Tukey’s multiple comparison test between the WT and NASP data points. In the figure panels, ns represents nonsignificant difference while *P < 0.05, **P < 0.01, and ***P < 0.001. Refer to the image caption for details. Panel A shows line graphs of H3.2-Dendra2 total intensity for nuclear cycles 11-13 in WT embryos, with time (min) on the x-axis and H3.2-Dendra2 total intensity (AU) on the y-axis. Panel B shows similar line graphs for NASP-deficient embryos. Panel C is a scatter plot comparing the nuclear import rates of H3.2-Dendra2 in WT (blue) and NASP-deficient (red) embryos, with nuclear cycle on the x-axis and H3.2-Dendra2 import rate (AU) on the y-axis. Panel D shows line graphs of H3.3-Dendra2 total intensity for nuclear cycles 11-13 in WT embryos, with time (min) on the x-axis and H3.3-Dendra2 total intensity (AU) on the y-axis. Panel E shows similar line graphs for NASP-deficient embryos. Panel F is a scatter plot comparing the nuclear import rates of H3.3-Dendra2 in WT (blue) and NASP-deficient (red) embryos, with nuclear cycle on the x-axis and H3.3-Dendra2 import rate (AU) on the y-axis. The graphs indicate the mean standard deviation (SD) and statistical significance is marked with asterisks.

H3 nuclear import is independent of NASP. (A) Total intensity curves of H3.2-Dendra2 for nuclear cycles (NC) 11–13 in WT embryos. (B) Total intensity curves of H3.2-Dendra2 for nuclear cycles (NC) 11–13 in NASP-deficient embryos. (C) Nuclear import rates of H3.2-Dendra2 in WT (blue) and NASP-deficient (red) embryos. Simulated import rate for H3.2 at 50% WT levels (black). N = 6 biological replicates. Data represent the mean ± standard deviation (SD). Statistical difference between groups were analyzed by employing a two-way ANOVA Tukey’s multiple comparison test between the WT and NASP data points. (D) Total intensity curves of H3.3-Dendra2 for nuclear cycles (NC) 11–13 in WT embryos. (E) Total intensity curves of H3.3-Dendra2 for nuclear cycles (NC) 11–13 in NASP-deficient embryos. (F) Nuclear import rates of H3.2-Dendra2 in WT (blue) and NASP-deficient (red) embryos. N = 6 experimental replicates. Data represent the mean ± standard deviation (SD). Statistical difference between groups were analyzed by employing a two-way ANOVA Tukey’s multiple comparison test between the WT and NASP data points. In the figure panels, ns represents nonsignificant difference while *P < 0.05, **P < 0.01, and ***P < 0.001.

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