Figure S3.
The top flow cytometry plots from left to right are as follows: A flow cytometry plot representing the relationship between CD45 and CD123. The horizontal axis is labeled CD45 BV510 and the vertical axis is labeled FSC-H. The plot shows hundreds of data points with a cluster of points in the lower right quadrant. A flow cytometry plot representing the relationship between CD33 and CD123. The horizontal axis is labeled CD33 Percp Cy5.5 and the vertical axis is labeled CD123 APC. The plot shows hundreds of data points with a cluster of points in the lower right quadrant. A flow cytometry plot representing the relationship between CD19 and CD34. The horizontal axis is labeled CD34 APC Cy7 and the vertical axis is labeled CD19 BV750. The plot shows hundreds of data points with a cluster of points in the upper left quadrant. A flow cytometry plot representing the relationship between CD3 and CD34. The horizontal axis is labeled CD34 APC Cy7 and the vertical axis is labeled CD3 BV785. The plot shows hundreds of data points with a cluster of points in the lower left quadrant. A flow cytometry plot representing the relationship between CD10 and CD34. The horizontal axis is labeled CD34 APC Cy7 and the vertical axis is labeled CD10 PE Cy7. The plot shows hundreds of data points with a cluster of points in the lower right quadrant. A flow cytometry plot representing the relationship between GFP and FSC-H. The vertical axis is labeled FSC-H and the horizontal axis is labeled GFP. The plot shows hundreds of data points with a cluster of points in the upper right quadrant. The bottom flow cytometry plots from left to right are as follows. A flow cytometry plot representing the relationship between CD3 and CD34. The horizontal axis is labeled CD34 APC Cy7 and the vertical axis is labeled CD3 BV785. The plot shows hundreds of data points with a cluster of points in the lower left quadrant. A flow cytometry plot representing the relationship between CD45RA and CD135. The horizontal axis is labeled CD45RA BV605 and the vertical axis is labeled CD135 PE. The plot shows hundreds of data points with a cluster of points in the lower right quadrant. A flow cytometry plot representing the relationship between CD10 and CD135. The horizontal axis is labeled CD10 PE Cy7 and the vertical axis is labeled CD135 PE. The plot shows hundreds of data points with a cluster of points in the lower right quadrant. A flow cytometry plot representing the relationship between GFP and FSC-H. The vertical axis is labeled FSC-H and the horizontal axis is labeled GFP. The plot shows hundreds of data points with a cluster of points in the upper right quadrant.
Representative flow cytometry gating for xenograft BM samples. The live singlet cells were gated for human CD45, and then, myeloid lineage was excluded (CD123–CD33−) prior to gating on CD19-negative (blue) or CD19-positive (red) populations. The CLP was further defined based upon CD19−CD3−CD34+CD10+ CD45RA+ CD135− (CD38+; data not shown). The hematogones (early B cell progenitors) were first captured in CD19+ (red) population and then further gated based upon CD19+CD3−CD34+CD38+CD10 (CD38+; data not shown). Percent GFP was positive in each population.