Figure 1.
IVIg products classified by immunogenic potential of HCP content. Characterization of host cell proteins (HCPs) by immunogenicity metrics. Each subplot represents one of the four products. Within each subplot, the individual HCPs are plotted based on their JanusMatrix Score (x-axis) and EpiMatrix Score (y-axis). The outline of each product’s HCP population is shown to facilitate visual comparison across subplots. EpiMatrix scores quantify epitope density normalized by protein length, enabling comparison across proteins of different sizes. JanusMatrix scores quantify the degree of cross-conservation between putative T cell epitopes and the human proteome. To obtain these scores, HCP proteins were parsed into overlapping 9-mer peptides, and each 9-mer was evaluated using the EpiMatrix algorithm for MHC class II-binding potential and using the JanusMatrix algorithm for sequence identity of TCR-facing residues to the human proteome. Refer to the image caption for details.

IVIg products classified by immunogenic potential of HCP content. Characterization of host cell proteins (HCPs) by immunogenicity metrics. Each subplot represents one of the four products. Within each subplot, the individual HCPs are plotted based on their JanusMatrix Score (x-axis) and EpiMatrix Score (y-axis). The outline of each product’s HCP population is shown to facilitate visual comparison across subplots. EpiMatrix scores quantify epitope density normalized by protein length, enabling comparison across proteins of different sizes. JanusMatrix scores quantify the degree of cross-conservation between putative T cell epitopes and the human proteome. To obtain these scores, HCP proteins were parsed into overlapping 9-mer peptides, and each 9-mer was evaluated using the EpiMatrix algorithm for MHC class II-binding potential and using the JanusMatrix algorithm for sequence identity of TCR-facing residues to the human proteome.

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