Panel A shows scatter plot of germinal center B cell numbers in draining lymph nodes with vertical axis number of germinal center B cells per million live dump negative cells and horizontal axis mouse genotypes. Panel B shows dot plot of combined variable heavy and variable light chain mutations comparing clonal and single sequences with vertical axis number of mutations and horizontal axis sequence categories and mouse genotypes. Panel C shows bar graph of immunoglobulin lambda variable gene usage with vertical axis frequency percentage and horizontal axis gene types across mouse genotypes. Panel D shows scatter plot of TM4 binding germinal center B cells with vertical axis number of TM4 binding germinal center B cells per million live dump negative cells and horizontal axis mouse genotypes.
Analysis of anti-HIV-1 sequential immunization. Related to Fig. 5. (A) Number of GC B cells in draining LNs after sequential immunizations on day 96. (B) Dot plot showing combined VH + VL gene nucleotide mutations among clonally expanded sequences compared with nonexpanded sequences (singlets). Each dot indicates an antibody VH + VL pair. Bars indicate the median. (C) Bar graphs showing Iglv gene usage in GC B cells. Top 10 most frequent genes ranked by the mean frequency in mM-only mice are shown. (D) Number of TM4-binding GC B cells in sequentially immunized mice on day 96 in draining LNs. Each dot represents a single mouse; bars indicate the mean ± SD unless otherwise indicated. ***P < 0.001; nonparametric one-way ANOVA P values are shown.