Figure S3.
Panel A shows a line graph of anti-RBD IgG antibodies in the serum of mice measured weekly from day 6 to day 97. The x-axis represents time in days, and the y-axis represents antibody levels in BT50. Thin lines represent individual mice, and bold lines represent group means. Panel B shows a bar graph, with the x-axis indicating different antibodies and the y-axis showing binding levels in BT50. Panel C shows a dot plot depicting somatic hypermutations in GC B cells on day 98. Each dot indicates one sequence, with the x-axis representing different groups (WT, M-only, mM-only) and the y-axis showing the number of mutations. Bars indicate median values, and significant differences are marked with asterisks.
Analysis of anti-spike prime boost responses. Related to Fig. 4. (A) ELISA quantification of anti-RBD IgG antibodies in the serum of mice from Fig. 4 measured weekly from day 6 to day 97. Thin lines represent single mice; bold lines represent the group mean. (B) Bar graph of recombinant antibodies binding to spike (top) or RBD (bottom) protein. Each bar represents one antibody. (C) Dot plot depicting somatic hypermutations in GC B cells on day 98 from Fig. 4. Each dot indicates one sequence. Bars indicate the median. P value of the two-sided Mann–Whitney U test is indicated. ****P < 0.0001.