Primary GC antibody sequences. (A) Pie charts depicting the distribution of antibody sequences pooled from four mice/genotype obtained 14 days after prime immunization from GC B cells. Inner circle numbers indicate the number of sequences analyzed for each genotype. The white section indicates nonexpanded sequences; colored or black pie slices are proportional to the number of clonally related sequences. The outlined black line indicates the percentages of cells in a clonal family. (B) Dot plot illustrating the percentage of clonally expanded sequences within each mouse. Each dot indicates one mouse. Bars indicate the mean. (C) Dot plot showing clonotype size distribution. Bars indicate the median. Each dot indicates a clone. (D) Dot plot showing Shannon’s entropy scores for the sequences depicted in A within each mouse. Bars indicate the median. (E) Dot plot showing combined VH + VL gene nucleotide mutations among sequences shown in A. Each dot indicates an antibody VH + VL pair. Bars indicate the median. (F) Dot plot depicting inverse Simpson’s index for sequences shown in A for each mouse. Each dot indicates one mouse. Bars indicate the median. (G) Bar graphs showing the relative abundance of mouse Ighv and Igkv gene usage among sequences shown in A. Top 10 most frequent genes ranked by the mean frequency in mM-only mice are shown. Bars indicate the mean ± SD. Statistics in A indicate chi-squared test with Monte Carlo P value simulation; P values were subsequently corrected using the Benjamini-Hochberg procedure. Statistics in B–F indicate the two-sided Mann–Whitney U test. ****P < 0.0001, ***P < 0.001, **P < 0.01.