Panel A shows a scatter bar plot of Salmonella Typhimurium burden in mesenteric lymph nodes at 5 days post-infection, normalized to Eubacteria 16S ribosomal RNA gene copies, where the y-axis represents gene copies per gram and the x-axis shows different treatment groups. Panel B shows a similar scatter bar plot for the liver. Panel C shows a scatter bar plot of Eubacteria 16S ribosomal RNA gene copies in mLN at 5 days post-infection, where the y-axis represents gene copies per gram and the x-axis shows different treatment groups. Panel D shows a similar scatter bar plot for the liver. Panel E shows a scatter bar plot of colon length in centimeters for different mouse groups at 5 days post-infection. Panel F shows a scatter bar plot of the number of CD4 positive and CD8 positive T cells in the small intestinal lamina propria at 5 days post-infection. Panel G shows a survival curve of survival percentage over days post-infection for different mouse groups, where the x-axis represents days post-infection and the y-axis represents survival percentage. Panel H shows a curve plot with data points of body weight variation percentage over days post-infection for different mouse groups, where the x-axis represents days post-infection and the y-axis represents body weight variation percentage. Each graph displays mean values with standard error of the mean, and statistical significance is indicated by asterisks.
Lingo4 deficiency enhances host resistance to S.typhimurium infection (related to Fig. 5). (A and B) S. typhimurium burden at 5 d.p.i. in mLN (A) and liver (B), normalized to Eubacteria 16S rRNA gene copies. Mean ± SEM, n = 4; representative of two experiments. (C and D) Eubacteria 16S rRNA gene copies in mLN (C) and liver (D) at 5 d.p.i. Mean ± SEM, n = 4; representative of two experiments. (E) Colon length of WT and Lingo4−/− mice at 5 d.p.i. Mean ± SEM, n = 6; two experiments combined. (F) Numbers of CD4+ and CD8+ T cells in siLP of WT and Lingo4−/− mice at 5 d.p.i. Mean ± SEM, n = 5; representative of two experiments. (G and H) Survival (G) and body weight variation (H) of WT and Lingo4−/− mice on Rag1−/− background during S.typhimurium infection with or without streptomycin pretreatment. Mean ± SEM, n = 8–9; two experiments combined. (A–H) Statistical significance was assessed using one-way ANOVA with Tukey’s post hoc test. *P < 0.05; ***P < 0.001. mLN, mesenteric LN.