Panel A shows a survival curve with the y-axis labeled Survival rate percent and the x-axis labeled Days post-infection. Panel B shows a curve plot with data points of body weight variation with the y-axis labeled Body weight variation percent and the x-axis labeled Days post-infection. Panel C shows a scatter bar plot with the y-axis labeled STM gene copies per gram normalized to Eubacteria and the x-axis labeled 5 days post-infection. Panel D shows representative hematoxylin and eosin sections of the small intestine and colon with no explicit axes. Panel E shows a scatter bar plot with the y-axis labeled Histopathological score and the x-axis labeled Small intestine and Colon. Panel F shows a scatter bar plot with the y-axis labeled Goblet cells frequency score and the x-axis labeled Small intestine and Colon. Panel G shows a scatter bar plot with the y-axis labeled Number of neutrophils and inflammatory monocytes and the x-axis labeled 5 days post-infection. Panel H shows flow cytometry plots and a scatter bar plot with the y-axis labeled Number of cells and the x-axis labeled ILC1, ILC2, and ILC3. Panel I shows flow cytometry plots and a scatter bar plot with the y-axis labeled Percentage of interleukin 22 positive ILC3s and the x-axis labeled mock and interleukin 23. Panel J shows a survival curve with the y-axis labeled Survival rate percent and the x-axis labeled Days post-infection. Panel K shows a curve plot with data points of body weight variation with the y-axis labeled Body weight variation percent and the x-axis labeled Days post-infection. Panel L shows a scatter plot with the y-axis labeled Salmonella Typhimurium colony forming units per gram and the x-axis labeled mock, anti interleukin 22, and recombinant interleukin 22.
Lingo4 −/− mice are more resistant to S.typhimurium infection due to reduced IL-22–mediated pathology. (A and B) Survival (A) and body weight change (B) of WT and Lingo4−/− mice infected with S. typhimurium, with or without streptomycin pretreatment. n = 18–20; three experiments combined. (C) Fecal S. typhimurium burden at 5 d.p.i., normalized to total Eubacteria 16S rRNA gene copies. Mean ± SEM, n = 4; representative of two experiments. (D–F) Representative H&E sections (D), histopathological score (E), and goblet cell frequency (F) at 5 d.p.i. Mean ± SEM, n = 4. Scale bars = 100 μm. (G and H) Numbers of neutrophils and inflammatory monocytes (G) and ILC3s (H) in siLP at 5 d.p.i. Mean ± SEM, n = 4–5; representative of two experiments. (I) Frequencies of IL-22+ ILC3s after IL-23 stimulation at 5 d.p.i. Mean ± SEM, n = 5. (J–L) Survival (J), body weight (K), and fecal S. typhimurium burden (L) in WT and Lingo4−/− mice treated with anti–IL-22 or rmIL-22 on days 0, 2, and 4 p.i. Mean ± SEM, n = 4. (A–L) Statistical significance was determined using unpaired two-tailed t test or one-way ANOVA with Tukey’s post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. rmIL-22, recombinant IL-22.