Panel A shows a schematic of the Clostridioides difficile infection model with antibiotic pretreatment including clindamycin and infection timeline (no explicit axes shown). Panel B shows a curve plot with data points of body weight variation over days post-infection, where the x-axis represents days post-infection and the y-axis represents body weight variation in percent. Panel C shows a curve plot with data points of mean clinical scores over days post-infection, where the x-axis represents days post-infection and the y-axis represents mean clinical score. Panel D shows a schematic of the Clostridioides difficile infection model without antibiotic pretreatment (no explicit axes shown). Panel E shows a curve plot with data points of body weight variation over days post-infection, where the x-axis represents days post-infection and the y-axis represents body weight variation in percent. Panel F shows a curve plot with data points of mean clinical scores over days post-infection, where the x-axis represents days post-infection and the y-axis represents mean clinical score. Panel G shows a survival curve of Citrobacter rodentium infection, where the x-axis represents days post-infection and the y-axis represents survival percentage. Panel H shows a curve plot with data points of body weight variation over days post-infection, where the x-axis represents days post-infection and the y-axis represents body weight variation in percent. Panel I shows a scatter plot of Citrobacter rodentium burden at 10 days post-infection, where the y-axis represents colony forming units per gram. Panel J shows a survival curve of Citrobacter rodentium infection, where the x-axis represents days post-infection and the y-axis represents survival percentage. Panel K shows a curve plot with data points of body weight variation over days post-infection, where the x-axis represents days post-infection and the y-axis represents body weight variation in percent. Panel L shows a scatter plot of Citrobacter rodentium burden at 10 days post-infection, where the y-axis represents colony forming units per gram. Panel M shows a curve plot with data points of body weight variation over days post-infection, where the x-axis represents days post-infection and the y-axis represents body weight variation in percent. Panel N shows a scatter plot of Citrobacter rodentium burden at 5, 10, and 15 days post-infection, where the x-axis represents time points and the y-axis represents colony forming units per gram.
Lingo4 deficiency increases susceptibility to C. difficile and C. rodentium (related to Fig. 4). (A–C) C. difficile infection (CDI) model schematic and outcomes: mice received 4-day antibiotic cocktail followed by i.p. clindamycin and infection with 108C. difficile (A); body weights (B) and clinical scores (C). Mean ± SEM, n = 8; two experiments combined. (D–F) CDI without antibiotic pretreatment (D), body weights (E), and clinical scores (F). Mean ± SEM, n = 8; two experiments combined. (G–I) Survival (G), body weight (H), and C. rodentium burden at 10 d.p.i. (I) in WT and Lingo4−/− mice on Rag1−/− background. Mean ± SEM, n = 4; representative of two experiments. (J–L) Survival (J), body weight (K), and C. rodentium burden at 10 d.p.i. (L) in WT and Lingo4−/− mice on Tcrb−/− background. Mean ± SEM, n = 6–9; two experiments combined. (M and N) Body weight (M) and C. rodentium burden at 5, 10, and 15 d.p.i. (N) in WT and Lingo4−/− mice on a Tcrd−/− background. Mean ± SEM; n = 5; representative of two experiments. (A–N) Statistical significance was assessed using unpaired two-tailed t tests or one-way ANOVA with Tukey’s post hoc test. *P < 0.05; **P < 0.01.