Figure 5.
Reduced AIDR112H PC formation from in vitro iGB cells. (A) PC differentiation in iGB cultures. Schematics of B cells cultured on 40LB cells and representative FACS plots for PB differentiation (CD138+ and IRF4hiPAX5lo cell population). (B) CD138+ PB cells from day 0 to day 7. FACS plots and quantification, day 4 (P < 0.0001), day 5 (P < 0.0001), and day 7 (P = 0.0009) (n = 9–11, four experiments). (C) IRF4hiPAX5lo cells from day 0 to day 7. FACS plots and quantification, day 4 (P = 0.016), day 5 (P = 0.033), and day 7 (P = 0.0001) (n = 13–22, five experiments). (D) Expression of IRF4 and PAX5 of iGB cells. Overlay of histograms of IRF4 and PAX5 acquired by flow cytometry. Quantification, IRF4 MFI: P = 0.00082, nonsignificant difference for PAX5 MFI, unpaired t test (n = 4, one representative experiment). (E) ImageStream analysis of IRF4, PAX5, and FOXO1 in iGB cells on day 7. Representative images of PAX5+ and IRF4hiPAX5lo cells from the iGB culture. Images were acquired with ImageStream flow cytometry (10,000 cells acquired, two experiments). ND, nondetectable (no cells could be detected). Value and error bar: mean ± SD. *P < 0.05, ***P < 0.001, ****P < 0.0001. Refer to the image caption for details. Panel A: A schematic diagram shows the process of B cell culture and differentiation into plasmablasts. Flow cytometry plots illustrate the differentiation of plasmablasts (CD138 positive and IRF4hiPAX5lo cell population). Panel B: Flow cytometry plots and a bar graph show the percentage of CD138 positive plasmablasts from day 0 to day 7 in wild-type (WT) and AIDR112H mice. The bar graph quantifies these percentages, indicating significant differences at day 0, day 4, day 5, and day 7. Panel C: Flow cytometry plots and a bar graph display the percentage of IRF4hiPAX5lo cells from day 0 to day 7 in WT and AIDR112H mice. The bar graph quantifies these percentages, showing significant differences at day 4, day 5, and day 7. Panel D: Overlaid histograms show the expression of IRF4 and PAX5 in iGB cells, with a bar graph quantifying the mean fluorescence intensity (MFI) of IRF4 and PAX5, indicating a significant difference in IRF4 MFI. Panel E: Images from Imagestream analysis show the expression of IRF4, PAX5, and FOXO1 in iGB cells on day 7, with representative images of PAX5 positive and IRF4hiPAX5lo cells.

Reduced AID R112H PC formation from in vitro iGB cells. (A) PC differentiation in iGB cultures. Schematics of B cells cultured on 40LB cells and representative FACS plots for PB differentiation (CD138+ and IRF4hiPAX5lo cell population). (B) CD138+ PB cells from day 0 to day 7. FACS plots and quantification, day 4 (P < 0.0001), day 5 (P < 0.0001), and day 7 (P = 0.0009) (n = 9–11, four experiments). (C) IRF4hiPAX5lo cells from day 0 to day 7. FACS plots and quantification, day 4 (P = 0.016), day 5 (P = 0.033), and day 7 (P = 0.0001) (n = 13–22, five experiments). (D) Expression of IRF4 and PAX5 of iGB cells. Overlay of histograms of IRF4 and PAX5 acquired by flow cytometry. Quantification, IRF4 MFI: P = 0.00082, nonsignificant difference for PAX5 MFI, unpaired t test (n = 4, one representative experiment). (E) ImageStream analysis of IRF4, PAX5, and FOXO1 in iGB cells on day 7. Representative images of PAX5+ and IRF4hiPAX5lo cells from the iGB culture. Images were acquired with ImageStream flow cytometry (10,000 cells acquired, two experiments). ND, nondetectable (no cells could be detected). Value and error bar: mean ± SD. *P < 0.05, ***P < 0.001, ****P < 0.0001.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal