Panel A: U M A P plot showing I g D-M E 1 and I g D-M E 2 subclusters with A B C gene signature and marker expression differences. Panel B: Violin plots showing expression of genes T N F R S F 13 B, M S 4 A 1, C D 44, S O X 5, S S R 4, I F N G R 1, G S N, and F G R across subclusters. Panel C: Vertical bar graph showing enriched G O terms and pathways distinguishing I g D-M E 1 from I g D-M E 2 subclusters.
Tonsillar IgD-ME B cells encompass two transcriptional subfractions expressing distinct ABC-like, metabolic, and signaling properties. (A) Top: UMAP depicting the distribution of ABC-associated transcripts within IgD-ME 1 and IgD-ME 2 subclusters; bottom: ITGAX, CD69, FCGR2B, TBX21, TOX, and LTB expression by each subcluster. (B)TNFRSF13B, MS4A1, CD44, SOX5, SSR4, IFNGR1, GSN, and FGR expression by each subcluster. (C) Bar plots showing significantly enriched GO terms (adj. P <0.05) in IgD-ME 1 compared with IgD-ME 2 subcluster; GO terms were obtained by GSEA of published scRNA-seq data. NES, normalized enrichment score. Data show one experiment with multiple biological replicates. Significance was determined by the Wilcoxon signed-rank test (A, bottom, B). *P < 0.05 and ****P < 0.0001.