Panel A: Box plot comparing I g lambda percent expression in I g D-M E, naive, I g G-M E, and I g A-M E B cell subsets. Panel B: Box plot comparing T B X 21 expression in counts per minute in I g D-M E, naive, I g G-M E, and I g A-M E B cell subsets. Panel C: Box plot comparing T A C I expression in mean fluorescence intensity in I g D-M E, naive, I g G-M E, and I g A-M E B cell subsets. Panel D: Confocal image of tonsil stained for I g D in green, I g M in red, C X C R 3 in magenta, and nuclear D N A in blue. Inset shows I g D-M E B cell at 380 x magnification with and without color merging.
Tonsillar IgD-ME B cells show biased Igλ expression along with prominent TBX21 (T-BET), TACI, and CXCR3 expression. (A) Flow cytometry of Igλ on naive and ME B cell subsets (N = 4). (B)TBX21 expression by naive and ME B cell subsets (N = 3–6). (C) Flow cytometry of TACI on naive and ME B cell subsets (N = 4–8). (D) Confocal imaging of tonsil stained for IgD (green), IgM (red), CXCR3 (magenta), and nuclear DNA (blue). Inset, IgD-ME B cell upon digital magnification (380×) with and without color merging. Scale bars, 200 µm (left panel) and 5 µm (right panels). Error bars, SD. Data summarize multiple independent experiments (A and C), one experiment with different biological replicates (B), or show results from one representative of at least three independent experiments (D). Statistical significance was assessed with the Kruskal–Wallis test followed by a post hoc pairwise Mann-Whitney test (A–C). *P < 0.05.