A curve plot with data points showing V one half (V1 divided by 2) and slope factor over time after WSC in Naive DRG. The horizontal axis represents time after WSC in minutes, ranging from 0 to 15. The left vertical axis represents V one half (V1 divided by 2) in millivolts, ranging from minus 110 to minus 80. The right vertical axis represents the slope factor in millivolts, ranging from 4 to 11. The graph shows two data curves: one for V one half (V1 divided by 2) (purple circles) and one for the slope factor (purple squares). The V one half (V1 divided by 2) curve shows a downward trend, while the slope factor curve shows a slight upward trend. Another curve plot with data points showing V one half (V1 divided by 2) and slope factor over time after WSC in SNI DRG. The horizontal axis represents time after WSC in minutes, ranging from 0 to 15. The left vertical axis represents V one half (V1 divided by 2) in millivolts, ranging from minus 110 to minus 80. The right vertical axis represents the slope factor in millivolts, ranging from 7 to 11. The graph shows two data curves: one for V one half (V1 divided by 2) (purple circles) and one for the slope factor (purple squares). The V one half (V1 divided by 2) curve shows a downward trend, while the slope factor curve shows a slight downward trend.
In naive DRG neurons (left), which already have large OMDs, supplementation with water-soluble cholesterol (WSC) has no effect on the slope factor, VS, but increasingly suppresses V1/2 as free cholesterol levels increase within the plasma membrane. In neuropathic DRG neurons (right), which have smaller OMDs and lower free cholesterol levels, cholesterol supplantation reduces both V1/2 and VS, particularly in the first 5 min.