Panel A: Flow cytometry plots showing the frequency of R O R gamma t positive Treg cells in H h-specific C D 4 positive T cells from the mesenteric lymph nodes (m L N) and large intestine (L I) of mice treated with I g G 1 F c or C T L A 4-I g. The x-axis represents R O R gamma t-P E-e F 610 and the y-axis represents F O X P 3-F I T C. Panel B: Bar graph showing the percentage of R O R gamma t positive Tregs of H h positive C D 4 positive T cells in the m L N of mice treated with I g G 1 F c or C T L A 4-I g. The y-axis represents the percentage of R O R gamma t positive Tregs. Panel C: Bar graph showing the percentage of R O R gamma t positive Tregs of H h positive C D 4 positive T cells in the L I of mice treated with I g G 1 F c or C T L A 4-I g. The y-axis represents the percentage of R O R gamma t positive Tregs. Panel D: Flow cytometry plots showing the frequency of R O R gamma t positive Treg cells in H h-specific C D 4 positive T cells from the m L N and L I of mice treated with I g G 2 b or anti-C T L A 4. The x-axis represents R O R gamma t-P E-e F 610 and the y-axis represents F O X P 3-e F 450. Panel E: Bar graph showing the percentage of R O R gamma t positive Tregs of H h positive C D 4 positive T cells in the m L N of mice treated with I g G 2 b or anti-C T L A 4. The y-axis represents the percentage of R O R gamma t positive Tregs. Panel F: Bar graph showing the percentage of R O R gamma t positive Tregs of H h positive C D 4 positive T cells in the L I of mice treated with I g G 2 b or anti-C T L A 4. The y-axis represents the percentage of R O R gamma t positive Tregs.
CTLA-4 modulation engages B7-dependent regulation of RORγt + Treg cells. (A–C) (A) Flow cytometry plots of the Hh-specific CD4+ T cells (mLN, top panel; LI, bottom panel) and frequency of RORγt+ Treg cells in the (B) mLN and (C) LI of Hh-colonized WT mice treated with IgG1 Fc (n = 15) or CTLA4-Ig (n = 15). (D–F) (D) Flow cytometry plots of the Hh-specific CD4+ T cells (mLN, top panel; LI, bottom panel) and frequency of RORγt+ Treg cells in the (E) mLN and (F) LI of Hh-colonized WT mice treated with IgG2b (n = 14) or anti-CTLA4 (n = 14). The data in Fig. 2 are pooled from three independent experiments. The data are shown as means ± SEM; the statistics shown in B, C, E, and F were obtained by unpaired two-tailed Student’s t test. ***P < 0.001; ****P < 0.0001.