Panel A: Heatmaps showing differential expression of genes in T G F-beta receptor signaling pathway across C D 163 positive and negative macrophage subsets. Panel B: U M A P plots showing distribution of cells expressing lamina propria and serosal gene signatures. Panel C: U M A P feature plots showing expression of S m a d 7, T h b s 1, N r r o s, and C c l 2 across macrophage populations. Panel D: Immunofluorescence images and vertical bar graph showing p S M A D 3 expression in macrophages across L P and S slash M regions. Panel E: Immunofluorescence images and vertical bar graph showing T G F-beta 1 expression in macrophages across L P and S slash M regions.
TGF-β signalling pathways differ across sub-tissular locations of the small intestine. (A) Heatmaps showing expression profiles of genes listed under the TGF-β receptor signalling pathway GO term (GO-0007179) and identified as a DEG by bulk RNA-seq. (B) UMAP plots showing cells expressing LP (top) or serosal (bottom) genes from ImmGen datasets. (C) UMAP plots showing (top) expression level of Smad7 and Thbs1 and (bottom) Nrros and Ccl2. (D) Left: Representative immunofluorescence image of small intestine section from WT mice. DAPI (blue), IBA1 (red), and pSMAD3 (green). Right: Quantification of pSMAD3-expressing cells in the LP and S/M of the small intestine in WT mice. (E) Left: Representative immunofluorescence image of small intestine section from WT mice. DAPI (blue), IBA1 (red), and TGF-β1 (green). Right: Quantification of TGF-β1–expressing cells in the LP and S/M of the small intestine in WT mice. (D and E) Data (n = 4–5 per group) are pooled from three independent experiments. Scale bar = 100 μm. Error bars show mean ± SD. Statistical comparisons were performed with an unpaired t test with Welch’s correction for parametric data and a Mann–Whitney test for nonparametric data. **, P ≤ 0.01. UMAP, uniform manifold approximation and projection.