Panel A: Confocal microscopy images showing nuclei of parental and paclitaxel-induced polyploid H 1299 and H 441 cells with enlarged multinucleated structures. Panel B: Violin plots showing nuclear area distribution in pixels for parental and polyploid H 1299 and H 441 cells. Panel C: Vertical bar graph showing percentage of cells with single or multiple nuclei in parental and polyploid conditions. Panel D: Migration assay images showing Boyden chamber results for parental and polyploid H 1299 and H 441 cells with quantification. Panel E: Western blot showing p J N K, total J N K, and alpha-Tubulin levels in parental and polyploid cells. Panel F: Western blot showing p J N K, J N K, and alpha-Tubulin levels in polyploid H 1299 cells treated with N A C or S P 600125. Panel G: Migration assay images showing Boyden chamber results for cells treated with D M S O, N A C, or S P 600125 with quantification.
JNK signaling is required for the migration of induced poly-aneuploid mammalian cells. (A) Confocal micrographs showing the nuclei of parental and paclitaxel-induced polyploid H1299 and H441 cells. Scale bars: 10 µm. (B) Nuclear area in indicated groups. n = 100. (C) Percentage of cells with one or more nuclei. n = 100. (D) Boyden chamber migration assay of cells from B; migrated cells were imaged and quantified (graph shown). (E and F) Western blot analysis of pJNK and total JNK levels in parental and polyploid cells (E) or in polyploid H1299 cells treated with NAC or the JNK inhibitor SP600125 (F). (G) Boyden chamber migration assay of cells from F; migrated cells were imaged and quantified (graph shown). Cells were treated with 1 mM NAC (HY-B0215; MedChemExpress) or 5 μM SP600125 (S1460; selleckchem) for 8 h before functional assays. Source data are available for this figure: SourceData F8.