Panel A: Confocal microscopy images showing K D E L signal with G F P clones and D A P I comparing control, f z r, and r u x conditions. Panel B: Scatter plot showing percentage of clones with punctate K D E L signal across control, f z r overexpression, and r u x overexpression. Panel C: Confocal microscopy images showing l a c Z, f z r, and r u x overexpression clones with X b p 1 co-expression stained with D H E and Hoechst.
Elevated ER stress drives ROS production in induced polyploid cells. (A) Confocal images showing KDEL-RFP (red in merged images and LUT in separated channels) expression in fzr-OE or rux-OE GFP clones (green) compared with control (+). Samples were stained with DAPI (blue). The second to fifth columns show zoomed-in views of the white dashed rectangles in the first column. Scale bars: 20 μm. (B) Percentage of clones showing punctiform KDEL-RFP signals in control (Ctrl, n = 10), fzr-OE (n = 13), and rux-OE (n = 11) groups. Data are mean ± SEM, analyzed by one-way ANOVA across three groups. **** represents P value <0.0001. (C) Confocal images of lacZ-OE, fzr-OE + lacZ-OE, fzr-OE + xbp1-OE, or rux-OE + lacZ-OE or rux-OE + xbp1-OE clones (green) under hhts>GFP, stained with Hoechst (blue) and DHE (red). White dashed lines mark the A-P boundary. Scale bars: 20 μm.