Panel A: Confocal microscopy images showing G s t D-G F P reporter with f z r overexpression and antioxidant co-expression stained with M m p 1 and D A P I. Panel B: Confocal microscopy images showing G s t D-G F P reporter with r u x overexpression and antioxidant co-expression stained with M m p 1 and D A P I. Panel C: Scatter plot showing mean intensity quantification of G s t D-G F P in f z r overexpression clones with different conditions. Panel D: Scatter plot showing mean intensity quantification of G s t D-G F P in r u x overexpression clones with different conditions. Panel E: Confocal microscopy images showing T R E-R F P levels in f z r overexpression with antioxidant co-expression in wing discs. Panel F: Confocal microscopy images showing T R E-R F P levels in r u x overexpression with antioxidant co-expression in wing discs. Panel G: Scatter plot showing mean intensity quantification of T R E-R F P in f z r overexpression conditions. Panel H: Scatter plot showing mean intensity quantification of T R E-R F P in r u x overexpression conditions. Panel I: Confocal microscopy images showing G s t D-G F P expression in f z r and r u x overexpression clones with or without N A C treatment. Panel J: Scatter plot showing mean intensity quantification of G s t D-G F P under control and N A C treatment conditions. Panel K: Scatter plot showing mean intensity quantification of M m p 1 under control and N A C treatment conditions.
ROS-mediated JNK activation in induced polyploid cells. (A) Confocal images showing GstD-GFP expression in fzr-OE + lacZ-OE, fzr-OE + sod1-OE, or fzr-OE + cat-OE clones under Cits>RFP, stained with Mmp1 (gray) and DAPI (blue). Scale bars: 20 μm. (B) Confocal images showing GstD-GFP expression in rux-OE + lacZ-OE, rux-OE + sod1-OE, or rux-OE + cat-OE under Cits>RFP, stained with Mmp1 (gray) and DAPI (blue). Scale bars: 20 μm. (C) Quantification of mean intensity of GstD-GFP in fzr-OE + lacZ-OE (n = 33), fzr-OE + sod1-OE (n = 24), and fzr-OE + cat-OE (n = 22) clones. (D) Quantification of mean intensity of GstD-GFP in rux-OE + lacZ-OE (n = 54), rux-OE + sod1-OE (n = 44), and rux-OE + cat-OE (n = 54) clones. (E) Confocal images showing TRE-RFP level in fzr-OE + lacZ-OE, fzr-OE + Sod1-OE, or fzr-OE + Cat-OE wing discs. Scale bars: 20 μm. (F) Confocal images showing TRE-RFP levels in rux-OE + lacZ-OE, rux-OE + sod1-OE, or rux-OE + cat-OE wing discs. Scale bars: 20 μm. (G) Quantification of mean intensity of TRE-RFP in fzr-OE + lacZ-OE (n = 26), fzr-OE + Sod1-OE (n = 37), or fzr-OE + Cat-OE (n = 28) wing discs. (H) Quantification of mean intensity of TRE-RFP in rux-OE + lacZ-OE (n = 35), rux-OE + Sod1-OE (n = 30), or rux-OE + Cat-OE (n = 31) wing discs. Data in C, D, G, and H are presented as mean ± SEM, analyzed by one-way ANOVA. **** represents P value <0.0001. (I) Confocal images showing GstD-GFP expression in fzr-OE or rux-OE clones fed with either normal food or NAC-containing food. Samples were stained with Mmp1 (gray) and DAPI (blue). White dashed outlines indicate polyploid clones. The second to sixth rows show zoomed-in views of the white dashed rectangles. Scale bars: 20 μm. (J) Quantification of mean intensity of GstD-GFP in fzr-OE or rux-OE clones under standard conditions (n = 27 for fzr-OE and n = 22 for rux-OE) or following NAC feeding (n = 26 for fzr-OE and n = 17 for rux-OE). (K) Quantification of mean intensity of Mmp1 in fzr-OE or rux-OE clones under standard conditions (n = 17 for fzr-OE and n = 9 for rux-OE) or following NAC feeding (n = 27 for fzr-OE and n = 15 for rux-OE). Data in J and K are presented as mean ± SEM and analyzed using an unpaired two-tailed t test in two groups. ** represents P value = 0.0037; **** represents P value <0.0001.