Figure 5.
JNK activation in induced polyploid cells is triggered by ROS. (A) Representative confocal images showing the expression of the oxidative stress reporter GstD-GFP (green) in control (+), fzr-OE, and rux-OE wing discs. RFP marks the expression domain (red), and DAPI stains nuclei (blue). Magnified views of the boxed regions (white dashed rectangles) are shown in subsequent columns. (B) Confocal images of wing discs with fzr-OE clones co-expressing lacZ-OE, Sod1-OE, or Cat-OE (GFP, green). Discs were stained with Mmp1 (gray) as a readout of JNK activity and DAPI (blue) to label nuclei. (C) Confocal images of wing discs with rux-OE clones co-expressing lacZ-OE, Sod1-OE, or Cat-OE (GFP, green). Tissues were stained with Mmp1 (gray) for JNK activity and DAPI (blue) for nuclei. In B and C, the top row shows whole discs; lower panels show magnified views of boxed regions. (D) Quantification of Mmp1 signal intensity in clones from fzr-OE + lacZ-OE (n = 34), fzr-OE + sod1-OE (n = 35), or fzr-OE + cat-OE (n = 35). (E) Quantification of Mmp1 signal intensity in clones from rux-OE + lacZ-OE (n = 24), rux-OE + sod1-OE (n = 32), or rux-OE + cat-OE (n = 32). Data are mean ± SEM analyzed by one-way ANOVA in three groups. **** represents P value ≤0.0001, *** represents P value = 0.0008, and ** represents P value = 0.0028. Scale bars: 20 μm. Refer to the image caption for details. Panel A: Confocal microscopy images showing G s t D-G F P oxidative stress reporter with R F P and D A P I in control, f z r, and r u x conditions. Panel B: Confocal microscopy images showing f z r overexpression clones with l a c Z, S o d 1, or C a t co-expression stained with M m p 1 and D A P I. Panel C: Confocal microscopy images showing r u x overexpression clones with l a c Z, S o d 1, or C a t co-expression stained with M m p 1 and D A P I. Panel D: Scatter plot showing M m p 1 intensity quantification in f z r overexpression clones with different co-expression conditions. Panel E: Scatter plot showing M m p 1 intensity quantification in r u x overexpression clones with different co-expression conditions.

JNK activation in induced polyploid cells is triggered by ROS. (A) Representative confocal images showing the expression of the oxidative stress reporter GstD-GFP (green) in control (+), fzr-OE, and rux-OE wing discs. RFP marks the expression domain (red), and DAPI stains nuclei (blue). Magnified views of the boxed regions (white dashed rectangles) are shown in subsequent columns. (B) Confocal images of wing discs with fzr-OE clones co-expressing lacZ-OE, Sod1-OE, or Cat-OE (GFP, green). Discs were stained with Mmp1 (gray) as a readout of JNK activity and DAPI (blue) to label nuclei. (C) Confocal images of wing discs with rux-OE clones co-expressing lacZ-OE, Sod1-OE, or Cat-OE (GFP, green). Tissues were stained with Mmp1 (gray) for JNK activity and DAPI (blue) for nuclei. In B and C, the top row shows whole discs; lower panels show magnified views of boxed regions. (D) Quantification of Mmp1 signal intensity in clones from fzr-OE + lacZ-OE (n = 34), fzr-OE + sod1-OE (n = 35), or fzr-OE + cat-OE (n = 35). (E) Quantification of Mmp1 signal intensity in clones from rux-OE + lacZ-OE (n = 24), rux-OE + sod1-OE (n = 32), or rux-OE + cat-OE (n = 32). Data are mean ± SEM analyzed by one-way ANOVA in three groups. **** represents P value ≤0.0001, *** represents P value = 0.0008, and ** represents P value = 0.0028. Scale bars: 20 μm.

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