Panel A: Confocal microscopy images showing m G F P marked clones with f z r or r u x overexpression stained with C i and D A P I. Panel B: Confocal microscopy images showing f z r overexpression with or without b s k D N or g r n d D N stained with M m p 1, C i, and D A P I. Panel C: Confocal microscopy images showing r u x overexpression with or without b s k D N or g r n d D N stained with M m p 1, C i, and D A P I.
JNK signaling is required for induced polyploid cell dynamics. (A) Representative confocal images of wing discs expressing fzr-OE or rux-OE under hh>Gal4, compared with the control (+). Clones are marked by mGFP (green). Discs were stained with Ci (red) and DAPI (blue). White arrowheads indicate polyploid cells that have crossed into the anterior compartment. (B) Representative confocal images of wing discs expressing fzr-OE alone or in combination with bskDN or grndDN, compared with control (+). Discs were stained for Mmp1 (gray), Ci (red), and DAPI (blue), with mGFP (green) marking the posterior compartment. (C) Representative images of rux-OE wing discs, with or without co-expression of bskDN or grndDN, compared with control (+), stained as in B. In both A and B, white dashed rectangles in the top panels highlight regions shown in the magnified images below. Scale bars: 20 μm.