Panel A: Confocal microscopy images showing wing discs with f z r or r u x overexpression under h h drives G a l 4 stained with m G F P, M m p 1, and D A P I. Panel B: Confocal microscopy images showing clones with f z r or r u x overexpression under A c t double arrow G F P with T R E-R F P and D A P I. Panel C: Time lapse microscopy images showing A c t double arrow G F P f z r overexpression clones with T R E-R F P signal dynamics over time. Panel D: Time lapse microscopy images showing A c t double arrow G F P r u x overexpression clones with T R E-R F P signal dynamics over time.
JNK signaling is activated in induced polyploid cells. (A) Representative confocal images of wing discs expressing fzr-OE or rux-OE under hh>Gal4, compared with control (+). Discs were stained for Mmp1 (gray) and DAPI (blue) and expressed membrane GFP (mGFP, green). White dashed boxes in the top panels highlight regions shown in the magnified images below. (B) Confocal images of FLP-out clones expressing fzr-OE or rux-OE under Act>>GFP control. TRE-RFP (red) reports JNK pathway activity; GFP marks clones (green), and DAPI (blue) marks nuclei. White dashed boxes in the top panels highlight regions shown in the magnified images below. (C) Time-lapse imaging of an Act>>GFP + fzr-OE clone (GFP, green) expressing TRE-RFP (gray). Red dashed regions mark TRE-RFP patterns, showing dynamic JNK activation over time. (D) Time-lapse imaging of an Act>>GFP + rux-OE clone (GFP, green) expressing TRE-RFP (gray). Red dashed regions mark TRE-RFP pattern, showing dynamic JNK activation over time. Scale bars: 20 μm.