Panel A: Confocal microscopy images showing egg chamber with D A P I nuclei, s l b o-G F P border cells, and T R E-R F P signal. Panel B: Confocal microscopy images showing T R E-R F P signal in control, f z r overexpression, and r u x overexpression polyploid clones. Panel C: Confocal microscopy images showing individual f z r and r u x overexpression clones with T R E-R F P and G F P signals. Panel D: Confocal microscopy images showing w g n R N A i with f z r overexpression stained with C i, M m p 1, and D A P I. Panel E: Confocal microscopy images showing w g n R N A i with r u x overexpression stained with C i, M m p 1, and D A P I.
JNK activation in induced polyploid cells. (A) Egg chamber stained with DAPI (blue). Border cells are marked by GFP (green), and TRE expression is labeled by RFP (gray). The first row shows the whole egg chamber, the second and third rows are zoomed-in views of the white dashed rectangles. Scale bars: 20 μm. (B) Confocal images of TRE-RFP (red) in control (+), fzr-OE and rux-OE polyploidy clones (green) in whole discs. Scale bars: 50 μm. (C) Individual clones of fzr-OE and rux-OE showing TRE-RFP signal. Scale bars: 10 μm. (D and E) Confocal images of wgn RNAi co-expressed with fzr-OE (D) or rux-OE (E), stained with Ci (red), Mmp1 (gray), and DAPI (blue). The second to sixth columns show zoomed-in views of the white dashed rectangles in the first column. White arrowheads indicate cells crossing the AP boundary. Scale bars: 20 μm.