Panel A: Flow cytometry plots showing D A P I ploidy distribution in control, f z r overexpression, and r u x overexpression G F P populations. Panel B: Confocal microscopy images showing R N A i effects with m G F P, C i, M m p 1, and D A P I with zoomed regions highlighted. Panel C: Confocal microscopy images showing f z r R N A i clones labeled with G F P and D A P I and L U T intensity visualization. Panel D: Confocal microscopy images showing r u x R N A i clones labeled with G F P and D A P I and L U T intensity visualization. Panel E: Confocal microscopy images showing C y c B R N A i with G F P, T R E-R F P, and D A P I including zoomed region details. Panel F: Confocal microscopy images showing h h t s driven m G F P with C y c B R N A i stained with M m p 1 and D A P I zoomed regions. Panel G: Confocal microscopy images showing f z r overexpression cells with D A P I highlighting D V and A P axis sectional views. Panel H: Confocal microscopy images showing r u x overexpression cells with D A P I highlighting D V and A P axis sectional views.
Induced polyploidy by fzr or rux overexpression results in basal cellular protrusions. (A) DAPI-based ploidy analysis of control (first column), fzr-OE (second column), and rux-OE (third column) wing discs. The first row shows ploidy in GFP- cells, while the second row shows ploidy in GFP+ cells. (B) Confocal images of fzr RNAi and rux RNAi samples stained with Mmp1 (gray), Ci (red), and DAPI (blue). The second and fourth rows present zoomed-in views of the white dashed rectangles in the first and third rows. (C) Clones expressing fzr RNAi (green in merged image and LUT in single image) stained with DAPI (blue). (D) Clones expressing rux RNAi (green in merged image and LUT in single image) stained with DAPI (blue). (E) Confocal images of Act>>GFP + TRE-RFP + CycB RNAi disc stained with DAPI (blue). The second to fifth columns (white dashed area) present zoomed-in view of the corresponding white dashed rectangle in the first column. (F) Confocal images of hhts>mGFP + CycB RNAi disc stained with DAPI (blue) and Mmp1 (gray). The second to sixth columns (white dashed line marked anterior-posterior boundary) show zoomed-in view of the white dashed rectangle in the first column. (G) Confocal images of fzr-OE (green) stained with DAPI (magenta) with dorsal-ventral (DV) axis view and AP axis view. (H) Confocal images of rux-OE (green) stained with DAPI (magenta), shown in both DV and AP axis views. White arrowheads indicate protrusions reaching the basal side. Scale bars: 20 μm.