Figure 3. Increased S. aureus –induced... | Rockefeller University Press

Figure 3.

Increased S. aureus–induced cutaneous infection in plasma kallikrein–deficient mice. WT and Klkb1−/− mice were injected subcutaneously with 1 × 109 CFU S. aureus bioluminescent strain Xen29 (n = 10 per group). (A) Representative S. aureus in vivo bioluminescence on a pseudocolor scale overlaid on top of a greyscale image of WT and Klkb−/− mice. (B) Bacterial counts as measured by in vivo bioluminescence of S. aureus demonstrated as mean total flux (photons per second) ± SEM in a logarithmic scale. (C) Mean total size of the skin lesion in cm2 ± SEM. (D–G) Bacterial burden observed in kidneys (D), spleens (E), lungs (F), and livers (G) of WT and Klkb1−/− mice at 5 days after infection compared with F12−/− mice (dashed lines). Values are CFUs ± SEM within the entire organ as determined by serial dilutions of tissue homogenates. *P < 0.05, **P < 0.01 versus WT mice. P values were determined using Student’s t test. Refer to the image caption for details.

Increased S. aureus–induced cutaneous infection in plasma kallikrein–deficient mice. WT and Klkb1^−/− mice were injected subcutaneously with 1 × 10⁹ CFU S. aureus bioluminescent strain Xen29 (n = 10 per group). (A) Representative S. aureus in vivo bioluminescence on a pseudocolor scale overlaid on top of a greyscale image of WT and Klkb^−/− mice. (B) Bacterial counts as measured by in vivo bioluminescence of S. aureus demonstrated as mean total flux (photons per second) ± SEM in a logarithmic scale. (C) Mean total size of the skin lesion in cm² ± SEM. (D–G) Bacterial burden observed in kidneys (D), spleens (E), lungs (F), and livers (G) of WT and Klkb1^−/− mice at 5 days after infection compared with F12^−/− mice (dashed lines). Values are CFUs ± SEM within the entire organ as determined by serial dilutions of tissue homogenates. *P < 0.05, **P < 0.01 versus WT mice. P values were determined using Student’s t test.