Panel A: Four scatter plots show B C R repertoire metrics including richness, diversity, somatic hypermutation, and clonality in P S M B 10 G 209 R versus control samples; x-axis represents sample groups, y-axis represents metric values. Panel B: Histograms show C D R 3 length distributions for T C R beta (T R B) and immunoglobulin heavy chain (I g H) across time points; x-axis represents C D R 3 length, y-axis represents frequency counts. Panel C: Principal component analysis (P C A) plots show V and V J gene usage patterns in B C R and T C R repertoires; x-axis represents P C 1, y-axis represents P C 2. Panel D: Bar plots show median frequencies of T R B V gene usage comparing control and P S M B 10 G 209 R; x-axis represents gene segments, y-axis represents frequency. Panel E: Bar plots show median frequencies of I G H V gene usage comparing control and P S M B 10 G 209 R; x-axis represents gene segments, y-axis represents frequency.
Immune repertoire metrics from bulk TCR and BCR sequencing. (A) BCR repertoire analysis of the PSMB10 G209R patient compared with age-matched controls (n = 10) shows similar overall metrics, including richness, diversity, somatic hypermutation, and clonality. (B) CDR3 length distributions in the TRB and IgH loci across three time points in the PSMB10 G209R patient compared with controls. (C) PCA of BCR and TCR V and VJ gene usage architecture. Statistics: Pillai–Bartlett trace from MANOVA across all principal components. (D and E) Median frequencies of TRBV and IGHV gene usage in the PSMB10 G209R patient compared with controls. The VJ gene usage in the TCR repertoire shows mild skewing relative to control samples. PCA, principal component analysis; MANOVA, multivariate analysis of variance.