Figure S4.
Panel A: This panel contains five histograms displaying the expression of K i-67, C D 25, I L-2, I F N gamma, and T N F alpha in both P S M B 10 G 209 R and control (P S M B 10 W T) peripheral blood cells, pre and post stimulation with C D 3 slash C D 28 beads. The x-axes represent the markers (K i-67, C D 25, I L-2, I F N gamma, T N F alpha), and the y-axes represent the count normalized to mode. The legend indicates four conditions: Control unstimulated, Control stimulated, P S M B 10 G 209 R unstimulated, and P S M B 10 G 209 R stimulated. Panel B: This panel contains two histograms showing phospho-S T A T 5 upregulation in C D 3 positive cells in response to I L-2, I L-7, and I L-15 stimulation in both P S M B 10 G 209 R and control peripheral blood. The x-axis represents p S T A T 5, and the y-axis represents the count normalized to mode. The legend indicates four conditions: unstimulated, I L-2 stimulated, I L-7 stimulated, and I L-15 stimulated. Panel C: This panel contains two histograms showing the expression of I L-7 R (C D 127) in P S M B 10 G 209 R T cells compared to control T cells, and in ex vivo generated T cells from P S M B 10 G 209 R H S C s compared to ex vivo generated T cells from control H S C s. The x-axis represents I L-7 R, and the y-axis represents the count normalized to mode. The legend indicates three conditions: unstained, control, and P S M B 10 G 209 R.
PSMB10 G209R T cells show normal immune phenotype and function. (A) Histograms displaying the expression of Ki-67, CD25, IL-2, IFNγ, and TNF in both PSMB10 G209R and PSMB10 WT peripheral blood before and after stimulation with CD3/CD28 beads, gated on CD4+ and CD8+ SP cells. (B) Phospho-STAT5 upregulation in CD3+ cells in response to IL-2, IL-7, and IL-15 stimulation in both PSMB10 G209R and PSMB10 WT peripheral blood. (C) Expression of IL-7R (CD127) in PSMB10 G209R T cells compared with PSMB10 WT T cells, and in ex vivo generated T cells from PSMB10 G209R HSCs compared with ex vivo generated T cells from PSMB10 WT HSCs.