Figure S1.
Panel A: Six vertical bar graphs show the relative expression of P S M B 8, P S M B 9, P S M B 10, P S M B 5, P S M B 6, and P S M B 7 normalized to R P L 19 (ribosomal protein L19). The x-axis labels are control, plus I F N beta (interferon beta), and plus I F N gamma (interferon gamma), while the y-axis represents relative expression. Blue bars represent P S M B 10 wild type (W T), and red bars represent P S M B 10 G 209 R. Panel B: Western blot images display the protein levels of P S M A 1–7 (proteasome subunit alpha 1–7), P S M B 5, P S M B 6, and beta-actin (beta-actin) as a loading control. Vertical bar graphs show the relative expression of P S M A 1–7, P S M B 5, and P S M B 6 normalized to W T control. The x-axis labels are control, plus I F N beta (interferon beta), and plus I F N gamma (interferon gamma), while the y-axis represents relative expression. Blue bars represent P S M B 10 W T, and red bars represent P S M B 10 G 209 R.
RNA and protein analysis of immuno- and standard proteasome subunits in PSMB10 WT and PSMB10 G209R skin fibroblasts. (A) RT-qPCR analysis of immunoproteasome subunits PSMB8-10 and the standard subunits PSMB5-7 with normalization to RPL19 (2-delta CT) upon stimulation with 75 U/ml IFNγ or 100 U/ml IFNβ for 24 h (n = 5, multiple unpaired t test). (B) Western blot analysis of proteasome subunits PSMA1-7, PSMB5, and PSMB6 with β-actin as the loading control (n = 3, multiple unpaired t test). The β-actin loading controls shown in B and Fig. 4, B and C are identical, as these panels were derived from the same experiment and immunoblot. Source data are available for this figure: SourceData FS1.