Figure S4.
Panel A shows cryo-E M data processing workflow leading to M i s 12 c M t w 1 c head 2 reconstruction, including particle picking, 2 D classification, focused classification, 3 D refinement, and multibody refinement. Panel B shows F S C plot indicating overall resolution of the reconstructed base body 2 map (approximately 6.5 Å). Panel C shows cryo-E M density map of base body 2 colored by local resolution, highlighting head 2 structural features. Panel D shows angular distribution of particle orientations contributing to base body 2 reconstruction.
Cryo-EM data processing to reconstruct Mis12c Mtw1c head 2 domain. (A) Cryo-EM data processing scheme used to reconstruct cryo-EM density maps containing continuous density for the Mis12cMtw1c head 2 domain, as described in the Materials and methods section. Blue and pink labeling abd boxes refers to processing steps in CryoSPARC and RELION 5.0, respectively. (B) FSC plot for base body 2. (C) Cryo-EM density map for base body 2 that was used to model the Mis12cMtw1c head 2 domain colored according to local resolution. (D) Angular distribution of views plot for the particles that contributed to the reconstruction of base body 2. FSC, Fourier shell correlation.