Panel A: U M A P embedding of hematopoietic cells sampled before, during, and after r h G-C S F administration and from normal controls (N C). Each dot represents a single cell. The axes are labeled U M A P 1 (horizontal) and U M A P 2 (vertical). Different colors represent different cell types. Panel B: Bar graph showing the relative proportions of each cell population before, during, and after r h G-C S F administration. The vertical axis is labeled Percentage and the horizontal axis lists different cell types. Different colors represent different conditions: Before G-C S F, During G-C S F, and After G-C S F. Panel C: Violin plots showing gene expression signature scores for B-cell lineage stages across conditions (C L P, Early Pro-B, Late Pro-B, Pre-B, Immature B, Mature B). The vertical axis is labeled Module score, and the horizontal axis lists different B-cell stages. Different colors represent different conditions: Before G-C S F, During G-C S F, After G-C S F, and N C.
Alterations in lineage-specific gene expression signatures across HSPC and B cell differentiation before, during, and after rhG-CSF administration. (A) Uniform Manifold Approximation and Projection embedding of hematopoietic cells sampled before, during, and after rhG-CSF administration and from NCs. Each dot represents a single cell. (B) Relative proportions of each cell population under before, during, and after rhG-CSF administration. (C) Violin plots showing gene expression signature scores for B cell lineage stages across conditions (CLP, early pro-B, late pro-B, pre-B, immature B, mature B). CLP, common lymphoid progenitors; CMP, common myeloid progenitors; GMP, granulocyte–macrophage progenitors; HSPC, hematopoietic stem and progenitor cells; MEP, megakaryocyte–erythroid progenitor; NK, natural killer cell; pDC, plasmacytoid dendritic cell; rhG-CSF, recombinant human granulocyte colony-stimulating factor.