Panel A: Violin plots show gene expression levels of developmental markers and transcription factors in the H S P C fraction. The horizontal axis labels are N O T C H 1, H O X B 4, G A T A 2, C S F 3 R, I K Z F 1, S P I 1, T C F 3, and A K 2. The vertical axis is labeled Expression levels. Three groups are compared: Before, After, and N C. Panel B: Box plots display module scores of I F N-related signaling pathways in various B-cell fractions. The horizontal axis labels are H S P C, C L P, Early_pro_B, Late_pro_B, Pre_B, Immature_B, and Mature_B. The vertical axis is labeled Module score. Three groups are compared: r h G-C S F(minus), r h G-C S F(plus), and NC. Panel C: A dot plot illustrates gene ratio and p.adjust values for signaling pathways associated with T-cell activation. The horizontal axis compares N C versus r h G-C S F(-) and N C versus r h G-C S F(plus). The vertical axis lists various signaling pathways.
Modulation of IFNγ-related signaling pathways following rhG-CSF administration. (A) Gene expression of developmental markers and transcription factors in the HSPC fraction was compared across the three groups. (B) Gene expression of IFNγ-related signaling pathways in each B cell fraction was compared across the three groups. In the B cell fractions, including HSPC and CLP, IFNγ-related signaling pathways were significantly downregulated in the rhG-CSF group. (C) We assessed signaling alterations in the T cell fraction, and found that signaling pathways associated with T cell activation were upregulated both with and without rhG-CSF administration, compared with the NC group. CLP, common lymphoid progenitors; GOBP, Gene Ontology Biological Process; HSPC, hematopoietic stem and progenitor cell; NC, normal control.