Panel A: Three scatter plots show the H S P C fraction in groups with and without r h G-C S F administration and normal controls (N C). The horizontal axis represents U M A P_1, and the vertical axis represents U M A P_2. H S P C cells are marked in red. Panel B: A scatter plot shows principal component analysis of gene expression profiles in the H S P C fraction across three groups. The horizontal axis represents U M A P_1, and the vertical axis represents U M A P_2. H S P C cells are marked in red, and non-H S P C cells are marked in blue. Panel C: A dot plot shows enrichment analysis of genes with significantly altered expression in the H S P C fraction of each group. The horizontal axis lists different pathways, and the vertical axis shows gene ratio and adjusted p-value. Panel D: A bar graph shows the number of up- and down-regulated genes with significant expression alteration between three groups. The horizontal axis lists group comparisons, and the vertical axis shows the number of genes. Panel E: A violin plot shows module scores based on gene sets with significantly decreased expression with r h G-C S F administration compared to without it. The horizontal axis lists different cell types, and the vertical axis shows module scores. Panel F: A dot plot shows gene set enrichment analysis of 244 genes, performed specifically on the H S P C cluster, showing a marked reduction in I F N-related signaling pathways. The horizontal axis represents gene ratio, and the vertical axis lists different pathways. Panel G: A scatter plot shows gene expression of I F N G in T-cells. The horizontal axis represents U M A P_1 and the vertical axis represents U M A P_2. Panel H: A bar graph compares I F N G gene expression in the T-cell fraction with and without r h G-C S F administration. The horizontal axis lists groups, and the vertical axis shows I F N G gene expression levels.
Downregulation of IFNγ signaling in the HSPC fraction in rhG-CSF(+) samples. (A) HSPC fraction in the with rhG-CSF, without rhG-CSF, and NC groups. (B) Principal component analysis of gene expression profiles in the HSPC fraction across three groups. (C) Enrichment analysis of genes with significantly altered expression in the HSPC fraction of each group. The HSPC fraction without rhG-CSF administration demonstrated upregulation of the NF-κB pathway and lymphocyte-related pathways. (D) Number of up- and downregulated genes with significant expression alteration between the three groups. (E) Module scores based on gene sets (n = 244) with significantly decreased expression with rhG-CSF administration compared to without it. (F) Gene set enrichment analysis of these 244 genes, performed specifically on the HSPC cluster, showing a marked reduction in IFNγ-related signaling pathways. (G and H) Gene expression plot of IFNG. (H) Comparison of IFNG gene expression in the T cell fraction with and without rhG-CSF administration. HSPC, hematopoietic stem and progenitor cells; IFNγ, interferon γ; NC, normal controls; NF-κB, nuclear factor κ-light-chain-enhancer of activated B cells; rhG-CSF, recombinant human granulocyte colony-stimulating factor.