Figure 2. Panel A: A scatter plot shows... | Rockefeller University Press

Figure 2.

$Gene expression alterations with and without rhG-CSF administration using scRNA-seq. (A) Cell-type annotation based on integrated scRNA-seq analysis of UPN1 and UPN2 with and without rhG-CSF administration, and NC samples. (B) Proportion of identified cell types, divided into three groups: rhG-CSF (−), rhG-CSF (+), and NC groups. (C–I) Module score for with and without rhG-CSF groups based on the significantly DEG sets for each cluster in NCs: HSPC score (C), CLP (D), early pro-B (E), late pro-B (F), pre-B (G), immature B (H), and mature B (I). Boxplots show module scores. Box = 25–75th percentile, line = median, whiskers = 1.5× interquartile range, points = outliers. (J) Comparison of CSF3R (G-CSF receptor) gene expression levels in HSPC and B cell fractions across three groups. CDP, common dendritic cell progenitor; CMP/GMP, common myeloid progenitors/granulocyte–monocyte progenitors; CLP, common lymphoid progenitors; CSF3R, colony-stimulating factor 3 receptor; HSPC, hematopoietic stem and progenitor cell; MEP, megakaryocyte–erythroid progenitor; NC, normal control; pDC, plasmacytoid dendritic cell; rhG-CSF, recombinant human granulocyte colony-stimulating factor; scRNA-seq, single-cell RNA-seq; UPN, unique patient number. Refer to the image caption for details.$ Panel A: A scatter plot shows cell type annotation based on integrated s c R N A-seq analysis of U P N 1 and U P N 2 with and without r h G-C S F administration, and normal control (N C) samples. Different colors represent various cell types. Panel B: A bar graph displays the proportion of identified cell types divided into three groups: r h G-C S F negative, r h G-C S F positive, and N C. The horizontal axis lists cell types, and the vertical axis shows the percentage. Panel C to I: Box plots show module scores for different cell types with and without r h G-C S F administration. The horizontal-axis lists cell types, and the vertical-axis shows module scores. Boxes represent the 25th to 75th percentiles, lines indicate medians, whiskers show 1.5 interquartile ranges, and points are outliers. Panel J: Line graphs compare C S F 3 R gene expression levels in H S P C and B-cell fractions across three groups. The horizontal-axis lists cell types, and the vertical-axis shows C S F 3 R expression levels.

Gene expression alterations with and without rhG-CSF administration using scRNA-seq. (A) Cell-type annotation based on integrated scRNA-seq analysis of UPN1 and UPN2 with and without rhG-CSF administration, and NC samples. (B) Proportion of identified cell types, divided into three groups: rhG-CSF (−), rhG-CSF (+), and NC groups. (C–I) Module score for with and without rhG-CSF groups based on the significantly DEG sets for each cluster in NCs: HSPC score (C), CLP (D), early pro-B (E), late pro-B (F), pre-B (G), immature B (H), and mature B (I). Boxplots show module scores. Box = 25–75th percentile, line = median, whiskers = 1.5× interquartile range, points = outliers. (J) Comparison of CSF3R (G-CSF receptor) gene expression levels in HSPC and B cell fractions across three groups. CDP, common dendritic cell progenitor; CMP/GMP, common myeloid progenitors/granulocyte–monocyte progenitors; CLP, common lymphoid progenitors; CSF3R, colony-stimulating factor 3 receptor; HSPC, hematopoietic stem and progenitor cell; MEP, megakaryocyte–erythroid progenitor; NC, normal control; pDC, plasmacytoid dendritic cell; rhG-CSF, recombinant human granulocyte colony-stimulating factor; scRNA-seq, single-cell RNA-seq; UPN, unique patient number.