The image contains multiple panels depicting toxicology studies of dual H I F-1 over 2 inhibitors in mice. Panel A shows a dose escalation scheme for mice administered vehicle or H I F inhibitors (1.21 S 9 N or 3.2.16) at indicated doses every 12 hours. Panels B and C display line graphs of body weight measurements over 14 and 40 days, respectively, with mean and standard deviation values. Panel D presents a box plot of blood urea nitrogen (B U N) levels, Panel E shows a bar graph of serum albumin levels, and Panel F depicts a box plot of serum aspartate aminotransferase (A S T) levels on day 40, with no significant differences noted. Panel G includes histological images of major organs stained with hematoxylin and eosin, showing representative sections from mice treated with vehicle, 1.21 S 9 N, or 3.2.16. Each panel is labeled with specific details and measurements, providing a comprehensive overview of the study's findings. All data is approximate.
Toxicology studies of dual HIFi . (A) Mice were administered vehicle or HIFi (1.21S9N or 3.2.16) at the indicated dose (in mg/kg OG) every 12 h for two doses followed by daily dose escalation. (B) Mice were administered vehicle or HIFi (180 mg/kg OG BID) for 14 days. BW was measured every 2–3 days (mean + SD, n = 3 mice per group). (C) Mice were treated with vehicle or either 1.21S9N or 3.2.16 (60 mg/kg OG BID) for 40 days. BW (mean ± SD; n = 5 mice) was measured every 2–3 days. (D–F) BUN (D), serum albumin (E), and serum AST (F) levels were determined on day 40. Data are presented as individual values and mean (n = 5); ns, no significant difference, one-way ANOVA with the Sidak post-test. (G) Mice were euthanized on day 40, and hematoxylin-and-eosin–stained sections of major organs were prepared. Representative images are shown. Scale bar, 100 μm.