The image contains multiple graphs and charts that illustrate the effect of H I F i therapy in breast cancer models. Panel A shows a timeline of E M T 6 cell injection and treatment schedules in mice. Panel B presents a Kaplan-Meier survival analysis with various treatment groups, including vehicle, I g G 2 a isotype-control antibody, anti-C T L A-4, 1.21 S 9 N, 3.2.16, and combinations. Panels C to H display individual tumor growth curves for each treatment group, indicating the number of mice achieving a durable complete response. Panel I shows m R N A expression levels analyzed by R T-q P C R, with significant differences marked. Panel J presents protein expression analyzed by immunoblot assays. Panels K and L depict tumor growth curves and survival rates of M M T V-P y M T mice treated with vehicle or 1.21 S 9 N. The graphs collectively demonstrate the impact of different treatments on tumor growth and survival in breast cancer models. All data is approximate.
Effect of HIFi therapy in BrCa models. (A) EMT6 cells were injected into the MFP of Balb/c mice, which were treated by IP injection of: vehicle or IgG2a isotype control antibody (200 μg Q3D); α-CTLA-4 (200 μg Q3D); 1.21S9N or 3.2.16 (40 mg/kg BID); or α-CTLA-4 + HIFi. (B) Kaplan–Meier analysis of mouse survival is shown. (C-H) For each treatment group, individual tumor growth curves are shown. The number of mice in each group that achieved a durable CR on day 100 (after tumor rechallenge on day 70) is indicated. (I and J) Mice with 150-mm3 EMT6 tumors in the MFP were treated with vehicle or either 1.21S9N or 3.2.16 (40 mg/kg BID) for 5 days, tumors were harvested 4 h after the last dose, and mRNA expression (I) and protein expression (J) were analyzed by RT-qPCR (mean + SD, n = 5; *P < 0.05 by one-way ANOVA with the Bonferroni post-test) and immunoblot assays, respectively. (K and L) Tumor growth curves for individual mice (K) and survival (L) of MMTV-PyMT mice treated by OG administration of either vehicle or 1.21S9N (60 mg/kg BID) for 6 wk starting on day 53 of life (n = 5 mice per group). Source data are available for this figure: SourceData F5.