Figure S7. Panel A shows representative... | Rockefeller University Press

Figure S7.

High-load mitochondrial ATP consumption is associated with intracellular Ca2+release kinetics in SA node myocytes. (A and B) Representative normalized confocal line-scan recordings of intracellular Ca2+ (red, A) and Mode 2 mito-iATP signals (green, B) from myocytes classified as high-load (left) or low-load (right). The dashed line indicates baseline fluorescence (F/F0 = 1). (C–E) Summary quantification of Ca2+ transient kinetics (N = 4 mice per group), including time to peak (C), decay t1/2 (D), and transient duration (E). (F–H) Summary quantification of Mode 2 mito-iATP transient kinetics. P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice. Refer to the image caption for details.

Panel A shows representative confocal line-scan recordings of intracellular calcium levels in red, with high-load on the left and low-load on the right. The dashed line indicates baseline fluorescence. Panel B shows representative confocal line-scan recordings of Mode 2 mitochondrial ATP signals in green, with high-load on the left and low-load on the right. The dashed line indicates baseline fluorescence. Panel C is a scatter plot summarizing the time to peak of calcium transients, with high-load on the left and low-load on the right. Panel D is a scatter plot summarizing the decay half-time of calcium transients, with high-load on the left and low-load on the right. Panel E is a scatter plot summarizing the duration of calcium transients, with high-load on the left and low-load on the right. Panel F is a scatter plot summarizing the time to peak of Mode 2 mitochondrial ATP transients, with high-load on the left and low-load on the right. Panel G is a scatter plot summarizing the decay half-time of Mode 2 mitochondrial ATP transients, with high-load on the left and low-load on the right. Panel H is a scatter plot summarizing the duration of Mode 2 mitochondrial ATP transients, with high-load on the left and low-load on the right. Each scatter plot includes large circles denoting per-animal means and small circles indicating individual biological replicates.

High-load mitochondrial ATP consumption is associated with intracellular Ca ²⁺ release kinetics in SA node myocytes. (A and B) Representative normalized confocal line-scan recordings of intracellular Ca²⁺ (red, A) and Mode 2 mito-iATP signals (green, B) from myocytes classified as high-load (left) or low-load (right). The dashed line indicates baseline fluorescence (F/F₀ = 1). (C–E) Summary quantification of Ca²⁺ transient kinetics (N = 4 mice per group), including time to peak (C), decay t_1/2 (D), and transient duration (E). (F–H) Summary quantification of Mode 2 mito-iATP transient kinetics. P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.