Figure S5. Panel A: Representative... | Rockefeller University Press

Figure S5.

High-gain mitochondrial ATP synthesis is associated with prolonged intracellular Ca2+transients. (A and B) Representative normalized confocal line-scan recordings of intracellular Ca2+ (red, A) and mito-iATP (green, B) from myocytes classified as high-gain (left) or low-gain (right). The dashed line indicates baseline fluorescence (F/F0 = 1). (C–E) Summary quantification of Ca2+ transient kinetics (N = 4 mice per group), including time to peak (C), decay t1/2 (D), and transient duration (E). (F–H) Summary quantification of Mode 1 mito-iATP transient kinetics, including time to peak (F), decay t1/2 (G), and transient duration (H). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice. Refer to the image caption for details.

Panel A: Representative normalized confocal line-scan recordings of intracellular calcium levels in high-gain and low-gain myocytes. The x-axis represents time in milliseconds, and the y-axis represents the fluorescence ratio F/F0. Panel B: Representative normalized confocal line-scan recordings of mitochondrial ATP levels in high-gain and low-gain myocytes. The x-axis represents time in milliseconds, and the y-axis represents the fluorescence ratio F/F0. Panel C: Scatter plot comparing the time to calcium peak between high-gain and low-gain myocytes. The y-axis represents time in milliseconds. Panel D: Scatter plot comparing the decay half-time of calcium transients between high-gain and low-gain myocytes. The y-axis represents time in milliseconds. Panel E: Scatter plot comparing the duration of calcium transients between high-gain and low-gain myocytes. The y-axis represents time in milliseconds. Panel F: Scatter plot comparing the time to mitochondrial ATP peak between high-gain and low-gain myocytes. The y-axis represents time in milliseconds. Panel G: Scatter plot comparing the decay half-time of mitochondrial ATP transients between high-gain and low-gain myocytes. The y-axis represents time in milliseconds. Panel H: Scatter plot comparing the duration of mitochondrial ATP transients between high-gain and low-gain myocytes. The y-axis represents time in milliseconds. Large circles denote per-animal means, and small circles indicate individual biological replicates.

High-gain mitochondrial ATP synthesis is associated with prolonged intracellular Ca ²⁺ transients. (A and B) Representative normalized confocal line-scan recordings of intracellular Ca²⁺ (red, A) and mito-iATP (green, B) from myocytes classified as high-gain (left) or low-gain (right). The dashed line indicates baseline fluorescence (F/F₀ = 1). (C–E) Summary quantification of Ca²⁺ transient kinetics (N = 4 mice per group), including time to peak (C), decay t_1/2 (D), and transient duration (E). (F–H) Summary quantification of Mode 1 mito-iATP transient kinetics, including time to peak (F), decay t_1/2 (G), and transient duration (H). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.