Figure 3.
Panel A is a Gene Ontology (GO) pathway analysis. The x-axis shows minus log10 (adjusted p-value), indicating statistical significance, and the y-axis lists enriched biological processes such as recombinant repair, homeostasis of number of cells, regulation of post-translational protein modification, leukocyte cell–cell adhesion, lymphocyte-mediated immunity, regulation of T cell activation, lymphocyte differentiation, regulation of hemopoiesis, cell–substrate adhesion, DNA recombination, and DNA double-strand break repair. Dot color represents genotype (Il33 KO plus N. b., Il17rb KO plus N. b., Crlf2 KO plus N. b.), and dot size indicates gene count. Panel B is a heatmap of selected differentially expressed genes. Columns represent individual samples grouped by genotype (Il33 KO plus N. b., Il17rb KO plus N. b., Crlf2 KO plus N. b., WT plus N. b.), and rows represent genes. The color scale shows z-scores ranging from minus 2 (blue, low expression) to plus 2 (pink or red, high expression). The heatmap highlights genotype-specific differences in gene expression during N. b. infection.
Blimp-1 fails to be upregulated under inflammatory conditions in alarmin-deficient ILC2s. (A) GO pathway analysis of differentially regulated genes from bulk RNA-seq of small intestinal ILC2s from Il33 KO, Il17rb KO, and Crlf2 KO compared with WT mice after N. brasiliensis infection. (B) Heatmap of the top differentially regulated genes from the different KOs 7 days after N. brasiliensis infection. N. b., N. brasiliensis. (A and B)n = 2–3 mice per group, showing technical replicates.