Figure S2.
The image contains three main panels labeled A, B, and C. Panel A shows three volcano plots displaying differentially expressed genes in Il33 KO, Il17rb KO, and Crlf2 KO mice relative to WT mice, all under N. b. infection. The x-axis represents Log 2 Fold Change, and the y-axis represents minus log 10 (p-value). Each dot corresponds to a gene, with significantly upregulated and downregulated genes separated on either side of the plot. The dashed lines indicate statistical and fold-change thresholds. Panel B presents KEGG pathway enrichment analysis of differentially expressed genes in infected KO mice compared with infected WT mice. The y-axis lists enriched pathways such as nucleocytoplasmic transport, DNA repair, viral carcinogenesis, cell cycle, spliceosome, PI3K-Akt signaling pathway, and pathways in cancer. The x-axis shows enrichment significance (minus log 10 adjusted p-value). Different colors represent Il33 KO N. b., Il17rb KO N. b., and Crlf2 KO N. b. Panel C contains multiple heatmaps of genes grouped by specific Gene Ontology (GO) terms, including recombinant repair, leukocyte cell-cell adhesion, regulation of hemopoiesis, homeostasis of number of cells, lymphocyte-mediated immunity, cell–substrate adhesion, regulation of post-translational protein modification, and lymphocyte differentiation. Rows represent genes, and columns represent individual samples (WT N. b. and each KO N. b.). The color scale (z-score) ranges from blue (low expression) to red (high expression), showing pathway-specific gene expression differences across genotypes during infection.
Transcriptomic analysis of alarmin-deficient ILC2s in N. brasiliensis infection . Related to Fig. 3. (A) Volcano plots of DEGs from the indicated infected KOs compared with infected WT mice. Mice were infected with N. brasiliensis for 7 days. (B) KEGG pathway analysis of infected KOs compared with infected WT mice. (C) Heatmaps of genes for pathways shown in Fig. 3 A. (A–C)n = 2–3 mice per group showing technical replicates.