Figure S2.
The five panels labeled from A to E present various analyses of K I F 3 A and K I F 3 B proteins. Panel A depicts a Hi Res-S E C chromatograph of the mouse brain S 3 fraction, probing the distribution of K I F 3 A, K I F 3 B, K A P 3, and T R I M 46, with retention volumes and marker sizes indicated. Panel B shows the prediction of coiled-coil domains for K I F 3 A and K I F 3 B, with graphs displaying sharpened coiled coil propensity, raw coiled coil propensity, and probabilities of core positions. Panel C presents a sequence alignment of the coiled-coil region of K I F 3 A and K I F 3 B among model organisms, highlighting heptad repeats with a probability greater than 0.2. Panel D shows experimental S A X S profiles and Guinier plots for the A B K and B B K complexes, with statistics provided for each. Panel E compares the pair distribution function P(r) of the A B K and B B K complexes, with distance measurements and fitting parameters detailed. The graphs are overlaid, with axes labeled appropriately for each type of data presented. All data is approximate.
Related to Figs. 3, 4 and 5. (A) HiRes-SEC chromatograph of the mouse brain S3 fraction probing the distribution of KIF3A, KIF3B, KAP3, and TRIM46. (B) Prediction of coiled-coil domains of KIF3A/B. cc: sharpened coiled coil propensity; raw_cc: raw coiled coil propensity; prob_a: the probability of a core position; prob_d: the probability of d core position. (C) Sequence alignment of the coiled-coil region of KIF3A/B among model organisms. The coiled-coil heptad repeats (with a probability >0.2) are highlighted with a background color. (D) Experimental SAXS profiles, Guinier plots, and the statistics of the ABK (in red) and BBK (in blue) complexes. (E) Comparison of pair distribution function P(r) of the ABK and BBK.