Panel A shows four line graphs of tumor growth across treatment replicates. The x-axis represents days post inoculation, and the y-axis represents tumor size in square millimeters. W Tplus D M S O shows rapid tumor growth, W T plus G A shows suppressed growth, K O plus D M S O shows moderate growth, and K O plus G A shows minimal growth. Panel B shows western blot detecting M e t t l 8-Flag and G A P D H. Panel C shows four line graphs of tumor growth in mutation model replicates. Panel D shows flow cytometry plots with three corresponding graphs. The data quantify frequencies of G z m B, I F N-γ, and Perforin positive O T-I cells. Panel E shows four line graphs of tumor growth under antibody treatments. Panel F shows flow cytometry plots with three corresponding graphs. The data quantify cytokine and cytotoxic molecule expression in tumor-infiltrating O T-I cells. Panel G shows four line graphs of tumor growth under G A and anti-P D-1 treatments. Panel H shows one scatter graph quantifying tumor-infiltrating O T-I cell numbers. Panel I shows flow cytrometry plots with two corresponding graphs. The plots quantify frequencies of C X 3 C R 1⁺ T c f 1 intermediate T E X cells.
Mettl8 inhibition promotes CD8 + T cell antitumor immunity and synergistically enhances PD-1 blockade. (A) Tumor growth of the mice in GA-treated adoptive-transferred model displayed in each replicate. n = 8 per group. (B) Western blot analysis of Mettl8-Flag and GAPDH in lysates from HEK293T cells transfected with pMIGR1-Empty, pMIGR1-Mettl8-WT, and pMIGR1-Mettl8-Mut plasmid. (C) Tumor growth curves for individual mice in the Mettl8-mutated mouse model. n = 4–6 per group. (D) Representative flow cytometry plots and cumulative data show the frequency of GzmB, IFN-γ, and perforin gated on tumor-infiltrating OT-I cells from the mice in C. n = 4–6 per group. (E) Tumor growth of the mice from the combined Mettl8 KO and anti–PD-1 treatment model displayed in each replicate. n = 8 per group. (F) Representative flow cytometry plots and cumulative data show the frequency of IFN-γ, GzmB, and perforin gated on tumor-infiltrating OT-I cells from the mice in E. n = 6 per group. (G) Tumor growth of the mice from GA and anti–PD-1 combined treatment model displayed in each replicate. n = 9 per group. (H) Cumulative data show the absolute number of tumor-infiltrating OT-I cells from the mice in G. (I) Representative flow cytometry plots cumulative data show the frequency and absolute number of CX3CR1+ Tcf1− Int-TEX cells gated on tumor-infiltrating OT-I cells from the mice in G. n = 7 mice per group. P value was calculated by two-tailed Student’s t test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Source data are available for this figure: SourceData FS5.