Panel A shows a U M A P plot showing clustering of tumor infiltrating T cells. Panel B shows a bubble plot showing marker gene expression across T cell clusters. Panel C shows U M A P feature plots showing expression of cluster marker genes. Panel D shows U M A P plot showing distribution of M e t t l 8 expression. Panel E shows bubble plot showing M e t t l 8 expression across clusters. Panel F shows U M A P plot showing clustering of tumor infiltrating C D 8 T cells. Panel G shows bubble plots showing signature genes in stem like clusters. Panel H shows bubble plot showing M e t t l 8 expression in clusters. Panel I shows heatmap showing differential gene expression between T cell states. Panel J shows diagram showing C R I S P R targeting strategy of M e t t l 8 allele. Panel K shows flow cytometry plots showing t d T o m a t o expression in T cells.
Mettl8 expression in CD8 + T cell subsets and generation of Mettl8-tdTomato-Flag mice. (A) UMAP plot showing the clustering of 9,557 OT-I cells infiltrating in the B16-OVA tumor. (B and C) Bubble (B) and UMAP (C) plots showing the character gene expression in each cluster. (D and E) UMAP (D) and bubble (E) plots showing the expression of Mettl8 in each cluster. (F) UMAP plot showing the clustering of CD8+ cells infiltrating in the mouse melanoma and colon adenocarcinoma tumor model. (G and H) Bubble plot showing signature gene (G) and Mettl8 (H) expression in effector/exhausted and stem-like clusters. (I) RNA-seq analysis was performed on tumor-infiltrating OT-I cells sorted into Ly108+ Tim3− TPEX and Tim3+ Ly108− TEX populations. Heatmaps depict signature genes associated with TPEX and TEX cells (Log2FC > 1, FDR < 0.05). (J) Targeting strategy of Mettl8 allele. (K) Expression of tdTomato in T cells of the spleens from Mettl8-tdTomato-Flag mice. n = 5–8 per group. Data are representative of two independent experiments. P value was calculated by one-way ANOVA; ****P < 0.0001.