The image consists of three main sections labeled A, B, and C. Section A displays micro C T renders of mouse brains from different genotypes, highlighting the presence or absence of lesions. It includes twelve K r i t 1 f l slash f l; i P i k 3 c a H 1047 R and twenty K r i t 1 f l slash f l; i P i k 3 c a H 1047 R; T e k f l slash f l mouse brains harvested on post-operative day 21. Section B shows representative microscopic images through cranial windows, visual images, and micro C T renders from two K r i t 1 f l slash f l; i P i k 3 c a H 1047 R; T e k f l slash f l mice with significant C C M lesions on post-operative day 21. It also includes H and E and Prussian Blue staining of the tissue sections. Section C presents similar types of images and staining from two K r i t 1 f l slash f l; i P i k 3 c a H 1047 R; T e k f l slash f l mice showing no typical C C M pathology on post-operative day 21. The scale bar in the images is 1 millimeter.
Genetic deletion of TIE2 prevents CCM formation. (A) microCT renders of all study subjects, including 12 Krit1fl/fl; iPik3caH1047R and 12 Krit1fl/fl; iPik3caH1047R; Tekfl/fl mouse brains, harvested on POD 21. Two pairs of representative microCT renders from this complete cohort are presented in Fig. 4 E with the corresponding brain specimens to demonstrate genotype-dependent differences in lesion burden. (B) Representative microscopic images through the cranial windows (top left panels), visual images (top center panels), and microCT renders (top right panels), paired with H&E and Prussian blue staining (bottom panels) from two Krit1fl/fl; iPik3caH1047R; Tekfl/fl mice showing significant CCM lesions on POD 21. (C) Representative microscopic images through the cranial windows (top left panels), visual images (top center panels), and microCT renders (top right panels), paired with H&E and Prussian blue staining (bottom panels) from two Krit1fl/fl; iPik3caH1047R; Tekfl/fl mice showing no typical CCM pathology on POD 21. Scale bar: 1 mm.