Figure 2.
Panel A shows flow cytometry plots of F O X P 3 and C T L A 4 expression in live lymphocytes and C D 3 positive C D 4 positive T cells from control and I P E X patient samples at baseline (peripheral blood mononuclear cells) and after day 5 activation. The first row shows C D 3 versus C D 4 gating of live lymphocytes, the second row shows C T L A 4 versus total F O X P 3 expression, and the third row shows full length F O X P 3 versus total F O X P 3 within C D 3 positive C D 4 positive T cells. Panel B shows western blot plots of F O X P 3 expression in activated C D 4 positive T cells including full length and delta 2 isoforms with beta actin as loading control along with bar plot quantification normalized to beta actin.
Activated patient CD4 + T cells express FOXP3. (A) Expression of FOXP3 in baseline and activated CD4+ T cells (first row) showing total FOXP3 (clone: 259D) and CTLA4 (second row) as well as total FOXP3 (clone: 259D) and full-length FOXP3 (clone: 150D) (third row) expression by flow cytometry in patient’s mother, healthy control, and patient (Pt) CD4+ T cells. (B) Western blot analysis of FOXP3 expression in the activated CD4+ T cells, showing the full-length and delta2 isoforms of the FOXP3 protein along with the quantification of the protein levels. Source data are available for this figure: SourceData F2.