The image contains multiple graphs comparing various metabolic and physiological parameters between wildtype and M y c t 1 e c K O mice. (A) A bar graph shows body composition as a percentage of total body mass, with fat and lean mass indicated for both wild-type and M y c t 1 e c K O mice, showing similar composition after 4 weeks on a high-fat diet. (B) A bar graph depicts water intake in milliliters per day, showing no significant difference between the two groups. (C) Scatter plots show energy expenditure in kilocalories per hour as a function of lean weight and body weight, indicating similar energy expenditure between wildtype and Myct1ecKO mice. (D) A bar graph shows fecal lipid content in milligrams per gram of feces, with no significant difference between the groups. (E) Multiple bar graphs display blood metabolic parameters, including glucose, triglycerides, free fatty acids, and cholesterol levels in the fed state, 24-hour fasted state, and 6-hour refed state, showing no significant differences. (F) A bar graph presents the results of an oral lipid tolerance test, indicating similar triglyceride responses between the groups. (G) A bar graph shows vascular permeability in different organs, including lungs, brain, kidneys, liver, and fat, with no significant difference between wildtype and M y c t 1 e c K O mice. (H) A schematic diagram outlines the experimental workflow for analyzing tumor growth after subcutaneous implantation of tumor cells. (I) A line graph quantifies tumor volume over time, showing reduced tumor growth in M y c t 1 e c K O mice. (J) A bar graph quantifies tumor weight at the end of the experiment, indicating reduced tumor weight in M y c t 1 e c K O mice. (K) Images of tumor sections stained for V e g f r 2 show tumor vasculature in wildtype and M y c t 1 e c K O mice. (L) A bar graph quantifies the V e g f r 2-positive tumor area, showing no significant difference between the groups.
Reduced adiposity in Myct1 ecKO mice is independent of angiogenesis, adipogenesis, and whole-body metabolic activity, related to Fig. 2. (A) Body composition is similar between wild-type and Myct1ecKO mice after 4 wk of HFD at the end of the metabolic cage experiment. n = 10 mice per genotype; mean ± SD; multiple Mann–Whitney tests, P > 0.05. (B) Water intake is similar between wild-type and Myct1ecKO mice. n = 10 mice per genotype; mean ± SD; Mann–Whitney test, P > 0.05. (C) Energy expenditure over lean and body weight is similar between wild-type and Myct1ecKO mice. n = 10 mice per genotype. (D)Myct1 ablation does not change fecal lipid content. Measurement of fecal lipids (mg) normalized to amount of feces collected (g). n = 4–5 mice per genotype; mean ± SD; Mann–Whitney test, P > 0.05. (E)Myct1 ablation does not alter blood metabolic parameters. Blood plasma was analyzed for concentrations of glucose, triglycerides, free fatty acids, and cholesterol from wild-type and Myct1ecKO mice of the long-term Myct1 deletion experiment: 46-wk-old females in fed state (left), 50-wk-old females after 24-h fasting (middle), and 50-wk-old males after 6-h fasting (right). n = 4–9 mice per genotype; mean ± SD; unpaired t test, P > 0.05. (F)Myct1 ablation does not alter tolerance to lipid. Oral lipid tolerance test performed after 6 h of fasting. n = 4–5 mice per genotype; mean ± SD; mixed effects model, P > 0.05. (G)Myct1 ablation does not affect vascular permeability. Evans blue tissue content was assessed 30 min after i.v. injection in wild-type and Myct1ecKO mice. n = 3 mice per genotype, 1 non-injected mouse shown as control; mean ± SD; multiple paired t test with Holm–Sidak’s correction for multiple comparisons, P > 0.05. (H) Experimental workflow for analysis of tumor angiogenesis. (I)Myct1 ablation reduces tumor growth. Quantification of LLC tumor volume from wild-type and Myct1ecKO mice from 6 to 15 days after implantation. n = 8 mice per genotype; mean ± SD; multiple unpaired t tests, P = 0.032 (*). (J)Myct1 ablation reduces tumor weight. Quantification of tumor weight from wild-type and Myct1ecKO LLC tumors at end of experiment (day 15). n = 8 mice per genotype; mean ± SD; unpaired t test, P = 0.036 (*). (K)Myct1 ablation does not affect tumor vascular density. Staining of LLC tumor sections for Vegfr2 (black). Scale bar, 1 mm. (L)Myct1 ablation does not affect tumor vascular density. Quantification of Vegfr2+ tumor area in wild-type and Myct1ecKO LLC size-matched tumors shown in K. n = 6 mice per genotype; mean ± SD; unpaired t test, P > 0.05. Icons used in E and H were created with BioRender.com and modified in Affinity.