Figure 4.
The image consists of four panels labeled A, B, C, and D. Panel A shows time-lapse fluorescence micrographs of a hypha co-expressing H s p 104-G F P and N o p 1-R F P during recovery from heat shock. Key events include budding, selective recruitment of H s p 104, disassembly, and cytoplasmic retention. Panel B provides additional examples showing preferential H s p 104 association with the pre-existing nucleolar compartment. New and old are labeled in the sections of H s p 104 N o p 1, while the sections of N o p 1 alone lack any labels. Panel C and D show kymographs from time-lapse sequences of hyphae co-expressing H s p 104-G F P and N o p 1-R F P, illustrating selective H s p 104 recruitment to the pre-existing compartment prior to the onset of mitosis. The kymographs highlight the disassembly of the new nucleolus at mitosis and the nuclear entry of H s p104, along with labels for new and old.
Hsp104 selectively associates with the preexisting nucleolar compartment before mitotic onset. (A) Time-lapse fluorescence micrographs of a hypha co-expressing Hsp104-GFP and Nop1-RFP during recovery from heat shock, showing nucleolar budding and selective recruitment of Hsp104 to the preexisting compartment prior to mitosis. (B) Additional examples showing preferential Hsp104 association with the preexisting nucleolar compartment. (C and D) Kymographs from time-lapse sequences of hyphae co-expressing Hsp104-GFP and Nop1-RFP, showing selective Hsp104 recruitment to the preexisting compartment prior to the onset of mitosis.