Figure 2.
The image consists of three panels labeled A, B, and C. Panel A shows time-lapse micrographs of a hypha co-expressing N o p1-R F P and Histone H 1-G F P under control conditions. The images depict typical nucleolar disassembly and segregation during nuclear division, with time points marked at 0, 4, 8, 10, and 11 minutes. Panel B displays corresponding time-lapse micrographs of a heat-shocked hypha, illustrating nucleolar remodeling prior to mitotic onset. The images are taken at 215, 275, 305, 307, and 318 minutes post-heat shock. Old nucleolus, new nucleolus, condensation, and daughter nuclei are labeled. Panel C presents time-lapse micrographs of a heat-shocked hypha expressing N o p 1-R F P, showing early nucleolar protrusion and bud expansion during recovery. The images are captured at 0, 88, 129, 202, 230, 250, and 252 minutes post-heat shock. Each panel highlights different stages and effects of nucleolar dynamics under varying conditions.
Heat shock induces remodeling of the nucleolus prior to mitotic onset. (A) Time-lapse micrographs of a hypha co-expressing Nop1-RFP and histone H1-GFP under control conditions, showing typical nucleolar disassembly and segregation during nuclear division. (B) Corresponding time-lapse micrographs of a heat-shocked hypha showing nucleolar remodeling prior to mitotic onset. Hyphae were heat shocked at 42°C for 45 min before imaging. (C) Time-lapse micrographs of a heat-shocked hypha expressing Nop1-RFP, showing apparent nucleolar budding and expansion during recovery. Images were acquired every 10 s; selected time points post heat shock are shown.